A Biomimetic Nano-Engineered Platform for Functional Tissue Engineering of Cartilage Superficial Zone

被引:20
作者
Dehghan-Baniani, Dorsa [1 ]
Mehrjou, Babak [2 ]
Chu, Paul K. [2 ]
Wu, Hongkai [1 ,3 ,4 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Div Biomed Engn, Hong Kong, Peoples R China
[2] City Univ Hong Kong, Dept Phys, Dept Mat Sci & Engn, Dept Biomed Engn,Kowloon, Tat Chee Ave, Hong Kong, Peoples R China
[3] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Peoples R China
[4] Guangzhou First Peoples Hosp, 1 Panfu Rd, Guangzhou, Guangdong, Peoples R China
关键词
articular cartilage superficial zones; chitosan hydrogels; drug delivery; nanopillars; silk; MESENCHYMAL STEM-CELLS; CHONDROGENIC DIFFERENTIATION; ARTICULAR-CARTILAGE; SYNOVIAL-FLUID; CHITOSAN; KARTOGENIN; SCAFFOLDS; SURFACE; FABRICATION; MORPHOLOGY;
D O I
10.1002/adhm.202001018
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Articular cartilage has limited regeneration capacity because of its acellular and avascular nature. Although tissue engineering has been shown to be life-saving, reforming cartilage zones required by the appropriate tissue functions are challenging. Herein, the need is addressed by designing and producing a nano-engineered structure mimicking the superficial zone (SZ) of articular cartilage. The substrate is based on silk with good mechanical properties in conjunction with nano-topographical and biochemical cues. Nanopillar arrays are produced on the silk surface to regulate the stem cell morphology rendering them with a flattened ellipsoidal shape that is similar to that of chondrocytes in the SZ of natural cartilage. The cell interactions are enhanced by nitrogen ion implantation and the biomolecule, kartogenin (KGN), is loaded to promote chondrogenesis of the stem cells and furthermore, a thermosensitive chitosan hydrogel is formed on the nanopatterned silk to produce rheological properties similar to those of a synovial fluid. Based on the in vitro results and mechanical properties, it is a desirable implantable smart structure mimicking the cartilage SZ with the ability of continuous drug release for cartilage regeneration.
引用
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页数:12
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共 55 条
  • [1] Improvement of early cell adhesion on Thai silk fibroin surface by low energy plasma
    Amornsudthiwat, Phakdee
    Mongkolnavin, Rattachat
    Kanokpanont, Sorada
    Panpranot, Joongjai
    Wong, Chiow San
    Damrongsakkul, Siriporn
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 111 : 579 - 586
  • [2] Preparation and characterization of a composite biomaterial including starch micro/nano particles loaded chitosan gel
    Baniani, Dorsa Dehghan
    Bagheri, Reza
    Solouk, Atefeh
    [J]. CARBOHYDRATE POLYMERS, 2017, 174 : 633 - 645
  • [3] Rheological Study of Viscosupplements and Synovial Fluid in Patients with Osteoarthritis
    Bhuanantanondh, Petcharatana
    Grecov, Dana
    Kwok, Ezra
    [J]. JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING, 2012, 32 (01) : 12 - 16
  • [4] Biofunctionalization of surfaces by energetic ion implantation: Review of progress on applications in implantable biomedical devices and antibody microarrays
    Bilek, Marcela M. M.
    [J]. APPLIED SURFACE SCIENCE, 2014, 310 : 3 - 10
  • [5] Free radical functionalization of surfaces to prevent adverse responses to biomedical devices
    Bilek, Marcela M. M.
    Bax, Daniel V.
    Kondyurin, Alexey
    Yin, Yongbai
    Nosworthy, Neil J.
    Fisher, Keith
    Waterhouse, Anna
    Weiss, Anthony S.
    dos Remedios, Cristobal G.
    McKenzie, David R.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (35) : 14405 - 14410
  • [6] Depth-Resolved Structural and Compositional Characterization of Ion-Implanted Polystyrene that Enables Direct Covalent Immobilization of Biomolecules
    Bilek, Marcela Milena Marie
    Kondyurin, Alexey
    Dekker, Stephen
    Steel, Bradley Clifton
    Wilhelm, Richard Arthur
    Heller, Rene
    McKenzie, David Robert
    Weiss, Anthony Steven
    James, Michael
    Moeller, Wolfhard
    [J]. JOURNAL OF PHYSICAL CHEMISTRY C, 2015, 119 (29) : 16793 - 16803
  • [7] Hydrophobic nanopillars initiate mesenchymal stem cell aggregation and osteo-differentiation
    Brammer, Karla S.
    Choi, Chulmin
    Frandsen, Christine J.
    Oh, Seunghan
    Jin, Sungho
    [J]. ACTA BIOMATERIALIA, 2011, 7 (02) : 683 - 690
  • [8] Fine-Tuning the Degree of Stem Cell Polarization and Alignment on Ordered Arrays of High-Aspect-Ratio Nanopillars
    Bucaro, Michael A.
    Vasquez, Yolanda
    Hatton, Benjamin D.
    Aizenberg, Joanna
    [J]. ACS NANO, 2012, 6 (07) : 6222 - 6230
  • [9] Silk hydrogel for cartilage tissue engineering
    Chao, Pen-Hsiu Grace
    Yodmuang, Supansa
    Wang, Xiaoqin
    Sun, Lin
    Kaplan, David L.
    Vunjak-Novakovic, Gordana
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2010, 95B (01) : 84 - 90
  • [10] Advanced Silk Fibroin Biomaterials for Cartilage Regeneration
    Cheng, Gu
    Davoudi, Zahra
    Xing, Xin
    Yu, Xin
    Cheng, Xin
    Li, Zubing
    Deng, Hongbing
    Wang, Qun
    [J]. ACS BIOMATERIALS SCIENCE & ENGINEERING, 2018, 4 (08): : 2704 - 2715