Phospholipases A2 in gastric juice of Helicobacter pylori- positive and negative individuals

Gastric juice is known to have phospholipase A(2) catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A(2), which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospholipase A(2) subtypes (pancreatic group I phospholipase A(2) and synovial-type group II phospholipase A(2)) in gastric juice and their relation to the presence of H. pylori in gastric mucosal biopsies in the same individuals. Gastric juice was collected from 29 individuals during gastroscopy. Biopsies were taken from the antrum and body of the stomach to determine the H. pylori status. We found catalytically active phospholipase A(2) and both group I and group II phospholipases A(2) in the gastric juice samples. The catalytic activity and the mass concentrations of group I and group II phospholipases A(2) correlated significantly with the pH value in gastric juice. The gastric juice of H. pylori positive individuals did not contain higher amounts of phospholipases A(2) than the juice of H. pylori negative individuals. Rather, the mass concentration of group II phospholipase A(2) in gastric juice seemed to be somewhat lower in individuals with H. pylori infection than in uninfected individuals. The results of the current study show that both group I and group II phospholipases A(2) are present in gastric juice. The main sources of phospholipases A(2) in gastric juice are probably other than H. pylori.