Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China

被引：29
作者：
Chen, Xiangpeng [1 ,2 ]
Xu, Baoping [2 ]
Guo, Jiayun [1 ,2 ]
Li, Changchong [3 ,4 ]
An, Shuhua [5 ]
Zhou, Yunlian [6 ]
Chen, Aihuan [7 ]
Deng, Li [8 ]
Fu, Zhou [9 ]
Zhu, Yun [1 ,2 ]
Liu, Chunyan [1 ,2 ]
Xu, Lili [1 ,2 ]
Wang, Wei [1 ,2 ]
Shen, Kunling [2 ]
Xie, Zhengde [1 ,2 ]
机构：
[1] Beijing Pediat Res Inst, Beijing Key Lab Pediat Resp Infect Dis, Beijing 100045, Peoples R China
[2] Capital Med Univ, Beijing Childrens Hosp, Natl Ctr Childrens Hlth, Key Lab Major Dis Children,Minist Educ,Natl Clin, Beijing 10045, Peoples R China
[3] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou, Peoples R China
[4] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China
[5] Childrens Hosp Hebei Prov, Shijiazhuang, Hebei, Peoples R China
[6] Zhejiang Univ, Sch Med, Childrens Hosp, Hangzhou, Zhejiang, Peoples R China
[7] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China
[8] Guangzhou Women & Childrens Med Ctr, Guangzhou, Guangdong, Peoples R China
[9] Chongqing Med Univ, Childrens Hosp, Chongqing, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
D O I：
10.1038/s41598-018-22826-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To identify the variations in fusion (F) protein gene of RSV in China, a molecular epidemiological study was conducted. A total of 553 RSV positive specimens were collected from 2338 pediatric patients hospitalized with community-acquired pneumonia during a multi-center study conducted during 2014-2016. A total of 252 samples (183 RSV A, 69 RSV B) were selected for F gene sequencing, and analyzed together with 142 F gene sequences downloaded from GenBank. The result showed that all the Chinese RSV A and RSV B strains could be divided respectively into three branches. Compared with RSV A/B prototype sequences respectively, there were significant amino acid (AA) mutations at multiple antigenic sites. For RSV A, changes were found at AA residues 122, 124, 125, 276 and 384, and for RSV B at AA residues 45, 116, 125, 172, 173 and 202. Variations in human histocompatibility leukocyte antigen-restricted CTL epitopes were also observed. In total, 56 amino acid differences for the complete F protein were found between the RSV A and B groups in China, while several mutations were only found in the RSV B strains during 2015-2016. The RSV F gene is relatively conserved in China, however, limited mutations are still occurring with time.
引用
收藏
页数：6
相关论文
共 17 条
[1]   HLA class I-restricted cytotoxic T-cell epitopes of the respiratory syncytial virus fusion protein [J].
Brandenburg, AH ;
de Waal, L ;
Timmerman, HH ;
Hoogerhout, P ;
de Swart, RL ;
Osterhaus, ADME .
JOURNAL OF VIROLOGY, 2000, 74 (21) :10240-10244
[2]   Viral Etiologies of Hospitalized Acute Lower Respiratory Infection Patients in China, 2009-2013 [J].
Feng, Luzhao ;
Li, Zhongjie ;
Zhao, Shiwen ;
Nair, Harish ;
Lai, Shengjie ;
Xu, Wenbo ;
Li, Mengfeng ;
Wu, Jianguo ;
Ren, Lili ;
Liu, Wei ;
Yuan, Zhenghong ;
Chen, Yu ;
Wang, Xinhua ;
Zhao, Zhuo ;
Zhang, Honglong ;
Li, Fu ;
Ye, Xianfei ;
Li, Sa ;
Feikin, Daniel ;
Yu, Hongjie ;
Yang, Weizhong .
PLOS ONE, 2014, 9 (06)
[3]   Antigenic Fingerprinting following Primary RSV Infection in Young Children Identifies Novel Antigenic Sites and Reveals Unlinked Evolution of Human Antibody Repertoires to Fusion and Attachment Glycoproteins [J].
Fuentes, Sandra ;
Coyle, Elizabeth M. ;
Beeler, Judy ;
Golding, Hana ;
Khurana, Surender .
PLOS PATHOGENS, 2016, 12 (04)
[4]   Efficacy and Optimization of Palivizumab Injection Regimens Against Respiratory Syncytial Virus Infection [J].
Gutfraind, Alexander ;
Galvani, Alison P. ;
Meyers, Lauren Ancel .
JAMA PEDIATRICS, 2015, 169 (04) :341-348
[5]   Sequence variability of the respiratory syncytial virus (RSV) fusion gene among contemporary and historical genotypes of RSV/A and RSV/B [J].
Hause, Anne M. ;
Henke, David M. ;
Avadhanula, Vasanthi ;
Shaw, Chad A. ;
Tapia, Lorena I. ;
Piedra, Pedro A. .
PLOS ONE, 2017, 12 (04)
[6]   Respiratory Syncytial Virus-Neutralizing Monoclonal Antibodies Motavizumab and Palivizumab Inhibit Fusion [J].
Huang, Kelly ;
Incognito, Len ;
Cheng, Xing ;
Ulbrandt, Nancy D. ;
Wu, Herren .
JOURNAL OF VIROLOGY, 2010, 84 (16) :8132-8140
[7]   Host Cell Entry of Respiratory Syncytial Virus Involves Macropinocytosis Followed by Proteolytic Activation of the F Protein [J].
Krzyzaniak, Magdalena Anna ;
Zumstein, Michael Thomas ;
Gerez, Juan Atilio ;
Picotti, Paola ;
Helenius, Ari .
PLOS PATHOGENS, 2013, 9 (04)
[8]   Structure and Function of Respiratory Syncytial Virus Surface Glycoproteins [J].
McLellan, Jason S. ;
Ray, William C. ;
Peeples, Mark E. .
CHALLENGES AND OPPORTUNITIES FOR RESPIRATORY SYNCYTIAL VIRUS VACCINES, 2013, 372 :83-104
[9]   Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus [J].
McLellan, Jason S. ;
Chen, Man ;
Joyce, M. Gordon ;
Sastry, Mallika ;
Stewart-Jones, Guillaume B. E. ;
Yang, Yongping ;
Zhang, Baoshan ;
Chen, Lei ;
Srivatsan, Sanjay ;
Zheng, Anqi ;
Zhou, Tongqing ;
Graepel, Kevin W. ;
Kumar, Azad ;
Moin, Syed ;
Boyington, Jeffrey C. ;
Chuang, Gwo-Yu ;
Soto, Cinque ;
Baxa, Ulrich ;
Bakker, Arjen Q. ;
Spits, Hergen ;
Beaumont, Tim ;
Zheng, Zizheng ;
Xia, Ningshao ;
Ko, Sung-Youl ;
Todd, John-Paul ;
Rao, Srinivas ;
Graham, Barney S. ;
Kwong, Peter D. .
SCIENCE, 2013, 342 (6158) :592-598
[10]   Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis [J].
Nair, Harish ;
Nokes, D. James ;
Gessner, Bradford D. ;
Dherani, Mukesh ;
Madhi, Shabir A. ;
Singleton, Rosalyn J. ;
O'Brien, Katherine L. ;
Roca, Anna ;
Wright, Peter F. ;
Bruce, Nigel ;
Chandran, Aruna ;
Theodoratou, Evropi ;
Sutanto, Agustinus ;
Sedyaningsih, Endang R. ;
Ngama, Mwanajuma ;
Munywoki, Patrick K. ;
Kartasasmita, Cissy ;
Simoes, Eric A. F. ;
Rudan, Igor ;
Weber, Martin W. ;
Campbell, Harry .
LANCET, 2010, 375 (9725) :1545-1555
12