Characterization of the intestinal microbiome of Hirschsprung's disease with and without enterocolitis

被引:51
作者
Yan, Zhilong [1 ]
Poroyko, Valeriy [2 ]
Gu, Song [1 ]
Zhang, Zheng [3 ]
Pan, Liya [4 ]
Wang, Jing [3 ]
Bao, Nan [1 ]
Hong, Li [4 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Surg, Shanghai, Peoples R China
[2] Univ Chicago, Dept Surg, Chicago Comer Childrens Hosp, Chicago, IL 60637 USA
[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Pediat Translat Med Inst, Shanghai, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Clin Nutr, Shanghai, Peoples R China
关键词
Hirschsprung's disease; Hirschsprung-associated enterocolitis; Metagenomics; PATHOGENESIS;
D O I
10.1016/j.bbrc.2014.01.104
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hirschsprung's disease (HD) is a congenital malformation of the gastrointestinal tract characterized by the absence of the distal enteric nervous system. Hirschsprung-associated enterocolitis (HAEC) is severe life threatening complication of HD. The disease pathogenesis is still unclear, but evidences suggest that the intestinal microbiota may play important role in the development of HD and HAEC. Because microbial abundance and diversity might differ in HD patients with enterocolitis, we sought to generate comparative metagenomic signatures to characterize the structure of the microbiome in HD patients with and without enterocolitis. Our experimental design is to enroll four HD patients (two with enterocolitis and two without enterocolitis). The microbiome was characterized by 16S rRNA gene, and the data obtained will be used to taxonomically classify and compare community structure among different samples. We found that the structure of the microbiome within HAEC patients are differ from those without enterocolitis. This study helps us to understand microbial contributions to the etiology of Hirschsprung-associated enterocolitis. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:269 / 274
页数:6
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