Effect of vitamin B-6 deficiency on an antibody production in mice

To investigate the effects of vitamin B-6 (B-6) deficiency on an antibody production in BALB/c mice, the production of specific immunoglobulin (Ig) E antibody against dinitrophenylated ovalbumin (DNP-OVA) mere measured by the methods of enzyme linked immunosorbent assay, The mice fed on on a B, deficient diet for it weeks were immunized intraperitoneally with DNP-OVA absorbed to aluminum hydroxide gel, The contents of anti DNP-IgE antibodies in sera of B-6 deficient mice significantly increased compared to that of control mice fed on a diet containing B-6, In addition, Interleukin-4, which was known to induce IgE production in allergic reactions from splenocytes of B-6 deficient mice, was approximately four-fold higher than that in control mice, According to the recovery test to the B-6 deficient mice, that is feeding the control diet for 21 days, all values in terms of the body, thymus, and spleen weight, total serum protein, IgG, and anti DNP-IgE content, regained almost the same levels as those of control, These results suggest that B-6 deficiency in mice would have relation to the stimulation of specific IgE antibody production against DNP-OVA.