High NUAK1 expression correlates with poor prognosis and involved in NSCLC cells migration and invasion

被引：50

作者：

Chen, Peng ^{[1
]}

Li, Kai

Liang, Yan

Li, Liqing

Zhu, Xiaolin ^{[2
]}

机构：

[1] Tianjin Med Univ Canc Inst & Hosp, Lung Canc Med Dept, Minist Educ, Key Lab Breast Canc Prevent & Therapy, Tianjin, Peoples R China

[2] Tianjin Med Univ Canc Inst & Hosp, Dept Ultrasound, Minist Educ, Key Lab Breast Canc Prevent & Therapy, Tianjin, Peoples R China

来源：

EXPERIMENTAL LUNG RESEARCH | 2013年 / 39卷 / 01期

关键词：

invasion; migration; NSCLC; NUAK1; prognosis; NF-KAPPA-B; LUNG-CANCER; MATRIX METALLOPROTEINASES; METASTASIS; ACTIVATION; MICROENVIRONMENT; PROGRESSION; PATHWAYS; ROLES; ARK5;

D O I：

10.3109/01902148.2012.744115

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Novel (nua) kinase family 1 (NUAK1) is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. In the present study, we investigated the role of NUAK1 in the migration and invasion of human nonsmall cell lung cancer (NSCLC) cells. Immunohistochemistry staining showed that the expression of NUAK1 correlated with the differentiation and stage of the carcinoma, as well as with lymph node metastasis. Inhibition of NUAK1 expression by small interference RNA severely impaired migration and invasion in A549 cells. In addition, we found that the knockdown of NUAK1 suppressed the expression of MMP-2 and MMP-9 and the activation of NF-kappa B, which can regulate the transcription of MMP-2 and MMP-9. Correspondingly, NUAK1 knockdown reduced lung metastasis in a xenograft mouse model of NSCLC. Taken together, our results suggest that NUAK1 plays an important role in NSCLC cell migration and invasion.

引用

页码：9 / 17

页数：9

共 25 条

[1] MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration [J].

Arora, Shilpi ;

Ranade, Aarati R. ;

Tran, Nhan L. ;

Nasser, Sara ;

Sridhar, Shravan ;

Korn, Ronald L. ;

Ross, Julianna T. D. ;

Dhruv, Harshil ;

Foss, Kristen M. ;

Sibenaller, Zita ;

Ryken, Timothy ;

Gotway, Michael B. ;

Kim, Seungchan ;

Weiss, Glen J. .

INTERNATIONAL JOURNAL OF CANCER, 2011, 129 (11) :2621-2631

[2] A combination of gefitinib and FOLFOX-4 as first-line treatment in advanced colorectal cancer patients. A GISCAD multicentre phase II study including a biological analysis of EGFR overexpression, amplification and NF-kB activation [J].

Cascinu, S. ;

Berardi, R. ;

Salvagni, S. ;

Beretta, G. D. ;

Catalano, V. ;

Pucci, F. ;

Sobrero, A. ;

Tagliaferri, P. ;

Labianca, R. ;

Scartozzi, M. ;

Crocicchio, F. ;

Mari, E. ;

Ardizzoni, A. .

BRITISH JOURNAL OF CANCER, 2008, 98 (01) :71-76

[3] Cytoplasmic Kaiso is associated with poor prognosis in non-small cell lung cancer [J].

Dai, Shun-Dong ;

Wang, Yan ;

Miao, Yuan ;

Zhao, Yue ;

Zhang, Yong ;

Jiang, Gui-Yang ;

Zhang, Peng-Xin ;

Yang, Zhi-Qiang ;

Wang, En-Hua .

BMC CANCER, 2009, 9

[4] New Signaling Pathways from Cancer Progression Modulators to mRNA Expression of Matrix Metalloproteinases in Breast Cancer Cells [J].

Delassus, Gregory S. ;

Cho, Hyojin ;

Eliceiri, George L. .

JOURNAL OF CELLULAR PHYSIOLOGY, 2011, 226 (12) :3378-3384

[5] Endothelin-1 (ET-1) promotes MMP-2 and MMP-9 induction involving the transcription factor NF-κB in human osteosarcoma [J].

Felx, Melanie ;

Guyot, Marie-Claude ;

Isler, Marc ;

Turcotte, Robert E. ;

Doyon, Jose ;

Khatib, Abdel-Majid ;

Leclerc, Severine ;

Moreau, Alain ;

Moldovan, Florina .

CLINICAL SCIENCE, 2006, 110 (06) :645-654

[6] Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting [J].

Gialeli, Chrisostomi ;

Theocharis, Achilleas D. ;

Karamanos, Nikos K. .

FEBS JOURNAL, 2011, 278 (01) :16-27

[7] Biomechanical Remodeling of the Microenvironment by Stromal Caveolin-1 Favors Tumor Invasion and Metastasis [J].

Goetz, Jacky G. ;

Minguet, Susana ;

Navarro-Lerida, Inmaculada ;

Jose Lazcano, Juan ;

Samaniego, Rafael ;

Calvo, Enrique ;

Tello, Marta ;

Osteso-Ibanez, Teresa ;

Pellinen, Teijo ;

Echarri, Asier ;

Cerezo, Ana ;

Klein-Szanto, Andres J. P. ;

Garcia, Ricardo ;

Keely, Patricia J. ;

Sanchez-Mateos, Paloma ;

Cukierman, Edna ;

Del Pozo, Miguel A. .

CELL, 2011, 146 (01) :148-163

[8] A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation [J].

Hou, X. ;

Liu, J-E ;

Liu, W. ;

Liu, C-Y ;

Liu, Z-Y ;

Sun, Z-Y .

ONCOGENE, 2011, 30 (26) :2933-2942

[9]

Huang Fen, 2010, Zhonghua Xin Xue Guan Bing Za Zhi, V38, P455

[10] Matrix Metalloproteinases: Regulators of the Tumor Microenvironment [J].

Kessenbrock, Kai ;

Plaks, Vicki ;

Werb, Zena .

CELL, 2010, 141 (01) :52-67

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