Overloaded and stressed: whole-cell considerations for bacterial synthetic biology

被引：165

作者：

Borkowski, Olivier ^{[1
,2
]}

Ceroni, Francesca ^{[1
,2
]}

Stan, Guy-Bart ^{[1
,2
]}

Ellis, Tom ^{[1
,2
]}

机构：

[1] Imperial Coll London, Ctr Synthet Biol & Innovat, London, England

[2] Imperial Coll London, Dept Bioengn, London, England

来源：

CURRENT OPINION IN MICROBIOLOGY | 2016年 / 33卷

基金：

英国工程与自然科学研究理事会;

关键词：

ESCHERICHIA-COLI; GENE-EXPRESSION; CIRCUIT PERFORMANCE; GROWTH; DESIGN; IMPLEMENTATION; PROTEINS; DYNAMICS; CLUSTER; MODEL;

D O I：

10.1016/j.mib.2016.07.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The predictability and robustness of engineered bacteria depend on the many interactions between synthetic constructs and their host cells. Expression from synthetic constructs is an unnatural load for the host that typically reduces growth, triggers stresses and leads to decrease in performance or failure of engineered cells. Work in systems and synthetic biology has now begun to address this through new tools, methods and strategies that characterise and exploit host construct interactions in bacteria. Focusing on work in E. coli, we review here a selection of the recent developments in this area, highlighting the emerging issues and describing the new solutions that are now making the synthetic biology community consider the cell just as much as they consider the construct.

引用

页码：123 / 130

页数：8