Protective cellular immunity: Cytotoxic T-lymphocyte responses against dominant and recessive epitopes of influenza virus nucleoprotein induced by DNA immunization

被引：109

作者：

Fu, TM ^{[1
]}

Friedman, A ^{[1
]}

Ulmer, JB ^{[1
]}

Liu, MA ^{[1
]}

Donnelly, JJ ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT VIRUS & CELL BIOL,W POINT,PA 19486

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 04期

关键词：

D O I：

10.1128/JVI.71.4.2715-2721.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA immunization offers a novel means to induce cellular immunity in a population with a heterogeneous genetic background. An immunorecessive cytotoxic T-lymphocyte (CTL) epitope in influenza virus nucleoprotein (NP), residues 218 to 226, was identified when mice were immunized with a plasmid DNA encoding a full-length mutant NP in which the anchor residues for the immunodominant NP147-155 epitope were altered, Mice immunized with wild-type or mutant NP DNA were protected from lethal cross-strain virus challenge, and the protection could be adoptively transferred by immune splenocytes, indicating the role of cell-mediated immunity in the protection. DNA immunization is capable of eliciting protective cellular immunity against both immunodominant and immunorecessive CTL epitopes in the hierarchy seen with virus infection.

引用

页码：2715 / 2721

页数：7

共 36 条

[1] TRANSGENIC MICE LACKING CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED T-CELLS HAVE DELAYED VIRAL CLEARANCE AND INCREASED MORTALITY AFTER INFLUENZA-VIRUS CHALLENGE [J].