Single-molecule analysis of RNA polymerase transcription

被引：98
作者：
Bai, Lu [1 ]
Santangelo, Thomas J.
Wang, Michelle D.
机构：
[1] Cornell Univ, Atom & Solid State Phys Lab, Dept Phys, Ithaca, NY 14853 USA
[2] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
来源：
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2006年 / 35卷
关键词：
initiation; elongation; kinetics; mechanism; pausing;
D O I：
10.1146/annurev.biophys.35.010406.150153
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.
引用
收藏
页码：343 / 360
页数：18
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