Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing

被引:7
作者
Abuzayed, Mazen A. [1 ]
Goktay, Mehmet [1 ]
Allmer, Jens [1 ]
Doganlar, Sami [1 ]
Frary, Anne [1 ]
机构
[1] Izmir Inst Technol, Dept Mol Biol & Genet, TR-35430 Urla Izmir, Turkey
关键词
Illumina sequencing; Genomic SSRs; Genetic diversity; Population structure; Vicia faba L; GENETIC DIVERSITY ANALYSIS; MICROSATELLITE MARKERS; SSR MARKERS; L; CONSTRUCTION; LOCI; MAP;
D O I
10.1007/s11105-016-1003-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Faba bean (Vicia faba L.) is an important food legume crop with a huge genome. Development of genetic markers for faba bean is important to study diversity and for molecular breeding. In this study, we used Next Generation Sequencing (NGS) technology for the development of genomic simple sequence repeat (SSR) markers. A total of 14,027,500 sequence reads were obtained comprising 4,208 Mb. From these reads, 56,063 contigs were assembled (16,367 Mb) and 2138 SSRs were identified. Mono and dinucleotides were the most abundant, accounting for 57.5 % and 20.9 % of all SSR repeats, respectively. A total of 430 primer pairs were designed from contigs larger than 350 nucleotides and 50 primers pairs were tested for validation of SSR locus amplification. Nearly all (96 %) of the markers were found to produce clear amplicons and to be reproducible. Thirty-nine SSR markers were then applied to 46 faba bean accessions from worldwide origins, resulting in 161 alleles with 87.5 % polymorphism, and an average of 4.1 alleles per marker. Gene diversity (GD) of the markers ranged from 0 to 0.48 with an average of 0.27. Testing of the markers showed that they were useful in determining genetic relationships and population structure in faba bean accessions.
引用
收藏
页码:61 / 71
页数:11
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