Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC 824 and its use in the formation of 1,2-propanediol

被引：0

作者：

Huang, KX ^{[1
]}

Rudolph, FB ^{[1
]}

Bennett, GN ^{[1
]}

机构：

[1] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1999年 / 65卷 / 07期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The K-m and V-max values for the substrate dihydroxyacetone phosphate were 0.53 mM and 1.56 mmol min(-1) mu g respectively. When E. coli glycerol dehydrogenase was coexpressed with methylglyoxal synthase in E. coli BL21 (DE3), 3.9 mM 1,2-propanediol was produced.

引用

页码：3244 / 3247

页数：4

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