Translocation of Arf1 to the secretory granules in rat parotid acinar cells

We investigated the interaction of ADP-ribosylation factor (Arf) with the secretory granules in rat parotid acinar cells. The 20.5-kDa small-molecular-mass GTP-binding protein in the cytosolic fraction of rat parotid acinar cells was identified as ADP-ribosylation factor1 by using a pan-Arf monoclonal antibody and isotype-specific polyclonal antibodies for Arf proteins 1, 3, 5, and 6, Incubation of the cytosolic fraction with isolated secretory granule membranes in the presence of GTP gamma S resulted in the translocation of Arf1 from the cytosolic fraction to the secretory granule membranes. The translocation was not observed in the presence of GDP beta S in place of GTP gamma S, indicating that the process is GTP-dependent, The immunoelectron microscopy experiment confirmed Arf1 is translocated to the secretory granules. A prior treatment of the granule membranes with trypsin inhibited the translocation of Arf1 at 2 mM Mg2+, but had no effect in the absence of Mg2+ (condition of spontaneous conversion of Arf-GDP to Arf-GTP), Thus, the trypsin-sensitive nucleotide exchange activity for Arf1 is probably associated with the secretory granule membranes, These results demonstrate Arf1 translocates to the secretory granules in rat parotid acinar cells. (C) 1998 Academic Press.