The distribution of active force generators controls mitotic spindle position

被引:270
作者
Grill, SW [1 ]
Howard, J
Schäffer, E
Stelzer, EHK
Hyman, A
机构
[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[2] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
D O I
10.1126/science.1086560
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
During unequal cell divisions a mitotic spindle is eccentrically positioned before cell cleavage. To determine the basis of the net force imbalance that causes spindle displacement in one-cell Caenorhabditis elegans embryos, we fragmented centrosomes with an ultraviolet laser. Analysis of the mean and variance of fragment speeds suggests that the force imbalance is due to a larger number of force generators pulling on astral microtubules of the posterior aster relative to the anterior aster. Moreover, activation of heterotrimeric guanine nucleotide-binding protein (G protein) alpha subunits is required to generate these astral forces.
引用
收藏
页码:518 / 521
页数:4
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