Electrochemiluminescence sensor using quantum dots based on a G-quadruplex aptamer for the detection of Pb2+

被引：38
作者：
Hai, Hong [1 ]
Yang, Feng [1 ]
Li, Jianping [1 ]
机构：
[1] Guilin Univ Technol, Guilin, Peoples R China
来源：
RSC ADVANCES | 2013年 / 3卷 / 32期
基金：
中国国家自然科学基金;
关键词：
SENSING APPLICATION; AQUEOUS-SOLUTION; DNA-MOLECULES; CDTE; ION; DNAZYMES; RECOGNITION; BIOSENSOR; RESONANCE; LEAD;
D O I：
10.1039/c3ra41616j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A layer of aptamer probes with hairpin structures was modified on the surface of Fe3O4-Au magnetic nanoparticles (Fe3O4-Au-Aptamer). The "stem-loop" structure of the hairpin aptamer was opened when adding Pb2+. Then a G-quadruplex was formed, exposing 3'-NH2, which bound to CdTe quantum dots. Thus the magnetic nanoparticles were separated and enriched on the surface of the magnetic controlled gold electrode (MCGE). By this method, a "turn-on" electrochemiluminescence (ECL) sensor for Pb2+ detection using quantum dots was constructed. The changes to the structure of the aptamer were characterized by cyclic voltammetry, AC impedance measurements, transmission electron microscopy and UV-Vis absorption spectroscopy. Pb2+ concentration and ECL signal presented a linear relationship in the range 2.0 x 10(-10)-1.0 x 10(28) M. The detection limit was 1.08 x 10(-11) M. In addition, the biosensor showed good selectivity, stability and reproducibility.
引用
收藏
页码：13144 / 13148
页数：5
相关论文
共 50 条
[41]   Single-molecule investigation of G-quadruplex using a nanopore sensor [J].
Shim, Jiwook ;
Gu, Li-Qun .
METHODS, 2012, 57 (01) :40-46
[42]   Label-free fluorescent sensor for lead ion detection based on lead(II)-stabilized G-quadruplex formation [J].
Zhan, Shenshan ;
Wu, Yuangen ;
Luo, Yanfang ;
Liu, Le ;
He, Lan ;
Xing, Haibo ;
Zhou, Pei .
ANALYTICAL BIOCHEMISTRY, 2014, 462 :19-25
[43]   G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection [J].
Xi, Hui ;
Juhas, Mario ;
Zhang, Yang .
BIOSENSORS & BIOELECTRONICS, 2020, 167
[44]   Rational design of an allosteric G-quadruplex aptamer probe for ultra-sensitive detection of melamine in milk [J].
Xie, Zhuohao ;
Li, Peng ;
Zhan, Haonan ;
Zhuang, Liuyan ;
Tao, Sijian ;
Deng, Liehua ;
Huang, Jiahao .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 210 :430-438
[45]   Electrochemiluminescence biosensor based on CdSe quantum dots for the detection of thrombin [J].
Li, Yanfen ;
Liu, Lili ;
Fang, Xuelin ;
Bao, Jianchun ;
Han, Min ;
Dai, Zhihui .
ELECTROCHIMICA ACTA, 2012, 65 :1-6
[46]   Label-free G-Quadruplex aptamer and Thioflavin-T based turn-off fluorescent detection of ethanolamine [J].
Bayrac, Abdullah Tahir ;
Acar, Yasemin .
DYES AND PIGMENTS, 2020, 172
[47]   G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic [J].
Liang, Gang ;
Liu, Xinhui .
MICROCHIMICA ACTA, 2015, 182 (13-14) :2233-2240
[48]   Nanopore detection of guanine derivatives based on vacancy G-quadruplex DNA [J].
Ren, Tong ;
Feng, Zhen ;
Jiang, Fang ;
Liu, Xingtong ;
Chen, Tingting ;
Guo, Yanli ;
Tian, Lei ;
Kang, Xiao-Feng ;
Yao, Fujun .
MICROCHEMICAL JOURNAL, 2025, 208
[49]   Ultrasensitive Pb2+ detection based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles [J].
Wang, Xin ;
Guo, Xiangqun .
ANALYST, 2009, 134 (07) :1348-1354
[50]   Highly Sensitive Detection of Pb2+ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry [J].
Yang, Rui ;
Fang, Tian-Fang ;
Lu, Li-Ping ;
Ye, Qing .
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2021, 49 (02) :309-317
12345