Electrochemiluminescence sensor using quantum dots based on a G-quadruplex aptamer for the detection of Pb2+

被引：38
作者：
Hai, Hong [1 ]
Yang, Feng [1 ]
Li, Jianping [1 ]
机构：
[1] Guilin Univ Technol, Guilin, Peoples R China
来源：
RSC ADVANCES | 2013年 / 3卷 / 32期
基金：
中国国家自然科学基金;
关键词：
SENSING APPLICATION; AQUEOUS-SOLUTION; DNA-MOLECULES; CDTE; ION; DNAZYMES; RECOGNITION; BIOSENSOR; RESONANCE; LEAD;
D O I：
10.1039/c3ra41616j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A layer of aptamer probes with hairpin structures was modified on the surface of Fe3O4-Au magnetic nanoparticles (Fe3O4-Au-Aptamer). The "stem-loop" structure of the hairpin aptamer was opened when adding Pb2+. Then a G-quadruplex was formed, exposing 3'-NH2, which bound to CdTe quantum dots. Thus the magnetic nanoparticles were separated and enriched on the surface of the magnetic controlled gold electrode (MCGE). By this method, a "turn-on" electrochemiluminescence (ECL) sensor for Pb2+ detection using quantum dots was constructed. The changes to the structure of the aptamer were characterized by cyclic voltammetry, AC impedance measurements, transmission electron microscopy and UV-Vis absorption spectroscopy. Pb2+ concentration and ECL signal presented a linear relationship in the range 2.0 x 10(-10)-1.0 x 10(28) M. The detection limit was 1.08 x 10(-11) M. In addition, the biosensor showed good selectivity, stability and reproducibility.
引用
收藏
页码：13144 / 13148
页数：5
相关论文
共 50 条
[21]   DNA aptamer-based detection of lysozyme by an electrochemiluminescence assay coupled to quantum dots [J].
Huang, Haiping ;
Jie, Guifen ;
Cui, Rongjing ;
Zhu, Jun-Jie .
ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (04) :816-818
[22]   G-quadruplex formation and sequence effect on the assembly of G-rich oligonucleotides induced by Pb2+ ions [J].
Liu, Wei ;
Zheng, Bin ;
Cheng, Sheng ;
Fu, Yan ;
Li, Wei ;
Lau, Tai-Chu ;
Liang, Haojun .
SOFT MATTER, 2012, 8 (26) :7017-7023
[23]   Preparation of AgInS2 quantum dots and their application for Pb2+ detection based on fluorescence quenching effect [J].
Xue, Tong ;
Shi, Yiyi ;
Guo, Jie ;
Guo, Meixian ;
Yan, Ya .
VACUUM, 2021, 193
[24]   Fluorescence detection of Pb2+ based on the DNA sequence functionalized CdS quantum dots [J].
Liu, Siyu ;
Na, Weidan ;
Pang, Shu ;
Su, Xingguang .
BIOSENSORS & BIOELECTRONICS, 2014, 58 :17-21
[25]   Cationic-perylene-G-quadruplex complex based fluorescent biosensor for label-free detection of Pb2+ [J].
Zhao, Xu-Hua ;
Gong, Liang ;
Wu, Yuan ;
Zhang, Xiao-Bing ;
Xie, Jun .
TALANTA, 2016, 149 :98-102
[26]   Capture of RNA G-quadruplex structures using an L-RNA aptamer [J].
Lam, Sin Yu ;
Umar, Mubarak Ishaq ;
Zhao, Haizhou ;
Zhao, Jieyu ;
Kwok, Chun Kit .
RSC CHEMICAL BIOLOGY, 2024, 5 (10) :1045-1051
[27]   Dual amplification in a fluorometric acetamiprid assay by using an aptamer, G-quadruplex/hemin DNAzyme, and graphene quantum dots functionalized with D-penicillamine and histidine [J].
Li Nana ;
Li Ruiyi ;
Sun Xiulan ;
Yang Yongqiang ;
Li Zaijun .
MICROCHIMICA ACTA, 2020, 187 (03)
[28]   Hemin/G-quadruplex based electrochemical sensor for highly sensitive detection of ATP in fish [J].
Wei, Jie ;
Li, Yumeng ;
Si, Qingyang ;
Xiao, Qiao ;
Chen, Qingmin ;
Jiao, Tianhui ;
Chen, Quansheng ;
Chen, Xiaomei .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2022, 916
[29]   Unlocking Pb2+ Sensing Potential in a DNA G-Quadruplex via Loop Modification with Fluorescent Chalcone Surrogates [J].
Johnson, Ryan E. ;
Murray, Makay T. ;
Roby, Dylan J. ;
Bycraft, Lucas J. ;
Churcher, Zachary R. ;
Yadav, Saanya ;
Johnson, Philip E. ;
Wetmore, Stacey D. ;
Manderville, Richard A. .
ACS SENSORS, 2023, 8 (12) :4756-4764
[30]   Electrochemiluminescent detection of Pb2+ by graphene/gold nanoparticles and CdSe quantum dots [J].
Lu, Liping ;
Guo, Linqing ;
Li, Jiao ;
Kang, Tianfang ;
Cheng, Shuiyuan .
APPLIED SURFACE SCIENCE, 2016, 388 :431-436
12345