Succinate semialdehyde dehydrogenase down-regulate γ-hydroxybutyric acid bindin deficiency does not sites in the mouse brain

被引：15

作者：

Mehta, AK

Gould, GG

Gupta, M

Carter, LP

Gibson, KM

Ticku, MK

机构：

[1] Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA

[2] Oregon Hlth & Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA

来源：

MOLECULAR GENETICS AND METABOLISM | 2006年 / 88卷 / 01期

关键词：

SSADH deficiency; GHB receptor; H-3]NCS-382; autoradiography; membrane homogenate binding;

D O I：

10.1016/j.ymgme.2005.11.012

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We investigated whether succinate semialdehyde dehydrogenase deficiency alters gamma-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [H-3]NCS-382 revealed no significant changes in the affinity (K-d), receptor density (B-max), or displacement potency (IC50) in various brain regions of Aldh5al(-/-) vs. Aldh5al(+/+) mice. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：86 / 89

页数：4

共 26 条

[1] Absence seizures in succinic semialdehyde dehydrogenase deficient mice: a model of juvenile absence epilepsy [J].

Cortez, MA ;

Wu, Y ;

Gibson, KM ;

Snead, OC .

PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2004, 79 (03) :547-553

[2]

Franklin K. B. J., 2013, PAXINOS FRANKLINS MO

[3] QUANTITATIVE FILM AUTORADIOGRAPHY FOR TRITIUM - METHODOLOGICAL CONSIDERATIONS [J].

GEARY, WA ;

TOGA, AW ;

WOOTEN, GF .

BRAIN RESEARCH, 1985, 337 (01) :99-108

[4]

Gibson K.M., 2001, METABOLIC MOL BASES, V8, P2079

[5] The clinical phenotype of succinic semialdehyde dehydrogenase deficiency (4-hydroxybutyric aciduria): Case reports of 23 new patients [J].

Gibson, KM ;

Christensen, E ;

Jakobs, C ;

Fowler, B ;

Clare, MA ;

Hammersen, G ;

Raab, K ;

Kobori, J ;

Moosa, A ;

Vollmer, B ;

Rossier, E ;

Iafolla, AK ;

Matern, D ;

Brouwer, OF ;

Finelstein, J ;

Aksu, F ;

Weber, HP ;

Bakkeren, JAJM ;

Gabreels, FJM ;

Bluestone, D ;

Barron, TF ;

Beauvais, P ;

Rabier, D ;

Santos, C ;

Umansky, R ;

Lehnert, W .

PEDIATRICS, 1997, 99 (04) :567-574

[6] Focal neurometabolic alterations in mice deficient for succinate semialdehyde dehydrogenase [J].

Gibson, KM ;

Schor, DSM ;

Gupta, M ;

Guerand, WS ;

Senephansiri, H ;

Burlingame, TG ;

Bartels, H ;

Hogema, BM ;

Bottiglieri, T ;

Froestl, W ;

Snead, OC ;

Grompe, M ;

Jakobs, C .

JOURNAL OF NEUROCHEMISTRY, 2002, 81 (01) :71-79

[7]

Gobaille S, 1999, J PHARMACOL EXP THER, V290, P303

[8] Quantitative autoradiographic analysis of the new radioligand [3H](2E)-(5-hydroxy-5,7,8,9-tetrahydro-6H-benzo[a][7]annulen-6-ylidene) ethanoic acid ([3H]NCS-382) at γ-hydroxybutyric acid (GHB) binding sites in rat brain [J].

Gould, GG ;

Mehta, AK ;

Frazer, A ;

Ticku, MK .

BRAIN RESEARCH, 2003, 979 (1-2) :51-56

[9] Therapeutic intervention in mice deficient for succinate semialdehyde dehydrogenase (γ-hydroxybutyric aciduria) [J].

Gupta, M ;

Greven, R ;

Jansen, EEW ;

Jakobs, C ;

Hogema, BM ;

Froestl, W ;

Snead, OC ;

Bartels, H ;

Grompe, M ;

Gibson, KM .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2002, 302 (01) :180-187

[10] Pharmacologic rescue of lethal seizures in mice deficient in succinate semialdehyde dehydrogenase [J].

Hogema, BM ;

Gupta, M ;

Senephansiri, H ;

Burlingame, TG ;

Taylor, M ;

Jakobs, C ;

Schutgens, RBH ;

Froestl, W ;

Snead, OC ;

Diaz-Arrastia, R ;

Bottiglieri, T ;

Grompe, M ;

Gibson, KM .

NATURE GENETICS, 2001, 29 (02) :212-216

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