The translocator protein ligand [18F]DPA-714 images glioma and activated microglia in vivo

被引：70

作者：

Winkeler, Alexandra ^{[1
,4
]}

Boisgard, Raphael ^{[1
,4
]}

Awde, Ali R. ^{[1
,4
]}

Dubois, Albertine ^{[1
,4
]}

Theze, Benoit ^{[1
,4
]}

Zheng, Jinzi ^{[1
,4
]}

Ciobanu, Luisa ^{[2
]}

Dolle, Frederic ^{[4
]}

Viel, Thomas ^{[3
]}

Jacobs, Andreas H. ^{[3
]}

Tavitian, Bertrand ^{[1
]}

机构：

[1] Univ Paris 11, INSERM, U1023, Lab Imagerie Mol Expt, F-91405 Orsay, France

[2] CEA, DSV, I2BM, LRMN, Gif Sur Yvette, France

[3] Westfalische Wilhelm Univ Munster WWU, EIMI, Munster, Germany

[4] CEA, I2BM, SHFJ, F-91400 Orsay, France

来源：

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | 2012年 / 39卷 / 05期

关键词：

Glioma; Translocator protein; TSPO; Microglia; Positron emission tomography; Peripheral benzodiazepine receptor; F-18] DPA-714; PERIPHERAL BENZODIAZEPINE-RECEPTOR; 18; KDA; BINDING-SITES; HUMAN-BRAIN; ALZHEIMERS-DISEASE; HUMAN ASTROCYTOMAS; AFFINITY BINDING; PET; EXPRESSION; RADIOLIGAND;

D O I：

10.1007/s00259-011-2041-4

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Purpose In recent years there has been an increase in the development of radioligands targeting the 18-kDa translocator protein (TSPO). TSPO expression is well documented in activated microglia and serves as a biomarker for imaging neuroinflammation. In addition, TSPO has also been reported to be overexpressed in a number of cancer cell lines and human tumours including glioma. Here we investigated the use of [F-18]DPA-714, a new TSPO positron emission tomography (PET) radioligand to image glioma in vivo. Methods We studied the uptake of [F-18]DPA-714 in three different rat strains implanted with 9L rat glioma cells: Fischer (F), Wistar (W) and Sprague Dawley (SD) rats. Dynamic [F-18]DPA-714 PET imaging, kinetic modelling of PET data and in vivo displacement studies using unlabelled DPA-714 and PK11195 were performed. Validation of TSPO expression in 9L glioma cell lines and intracranial 9L gliomas were investigated using Western blotting and immunohistochemistry of brain tissue sections. Results All rats showed significant [F-18]DPA-714 PET accumulation at the site of 9L tumour implantation compared to the contralateral brain hemisphere with a difference in uptake among the three strains (F > W > SD). The radiotracer showed high specificity for TSPO as demonstrated by the significant reduction of [F-18]DPA-714 binding in the tumour after administration of unlabelled DPA-714 or PK11195. TSPO expression was confirmed by Western blotting in 9L cells in vitro and by immunohistochemistry ex vivo. Conclusion The TSPO radioligand [F-18]DPA-714 can be used for PET imaging of intracranial 9L glioma in different rat strains. This preclinical study demonstrates the feasibility of employing [F-18]DPA-714 as an alternative radiotracer to image human glioma.

引用

页码：811 / 823

页数：13

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