Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification

被引：50

作者：

Dlauchy, D ^{[1
]}

Tornai-Lehoczki, J ^{[1
]}

Péter, G ^{[1
]}

机构：

[1] Univ Hort & Food Sci, Natl Collect Agr & Ind Microorganisms, H-1118 Budapest, Hungary

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1999年 / 22卷 / 03期

关键词：

ribotyping; 18S rDNA; yeast; identification; taxonomy;

D O I：

10.1016/S0723-2020(99)80054-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method has been developed to simplify the identification of yeast strains. We used the restriction fragment patterns of PCR amplified 18S rRNA-coding DNA with the neighbouring ITS1 region for differentiation and identification of 169 yeast strains representing 128 species associated mainly with food, wine, beer, and soft drinks. The amplicons were digested with four different four-base-cutting restriction enzymes. To construct a database of restriction fragment patterns, the gels have been scanned and analyzed using the Molecular Analyst Fingerprint 2.0 software. The use of four enzymes proved to be sufficient for strain identification.

引用

页码：445 / 453

页数：9

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