Protective effects of plicatin B on micronucleus induction in cultured human lymphocytes by different mutagens

被引：6

作者：

Eventi, Annalisa ^{[1
]}

Lubrano, Valter ^{[2
]}

Scarpato, Roberto ^{[3
]}

Turchi, Gino ^{[1
]}

机构：

[1] IBF CNR, Biochem & Mutagenesis Somat Cells Unit, I-56124 Pisa, Italy

[2] CNR, Inst Clin Physiol, I-56100 Pisa, Italy

[3] Univ Pisa, Dept Biol, Pisa, Italy

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2009年 / 47卷 / 01期

关键词：

Chalcone plicatin B; cytotoxicity; Micronucleus; ANTIOXIDANT ACTIVITY; IN-VITRO; ANTIMUTAGENIC ACTIVITY; BIOLOGICAL EVALUATION; DNA-DAMAGE; CHALCONES; BLEOMYCIN; MECHANISMS; FLAVONOIDS; CELLS;

D O I：

10.1016/j.fct.2008.10.017

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Among flavonoids, chalcones have been identified as interesting compounds having antioxidant, antimutagenic and antitumour properties. In this study we have evaluated the clastogenicity of plicatin B on human lymphocytes (whole blood and isolated lymphocytes) and its anticlastogenic activity, in the same cellular systems, using mitomycin C (MMC), radio-mimetic bleomycin (BL) and hydrogen peroxide (H2O2) as reference clastogens. Plicatin B per se resulted cytotoxic at high dose but non-clastogenic. The clastogenic effects induced by MMC and BL were significantly reduced (-33%) whereas with H2O2 the protective effect of plicatin B was observed (-62%) only at the greater H2O2 dose. The anticlastogen effects of plicatin B were effective when the chalcone was introduced in the culture, 1 h before the reference clastogens. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：124 / 128

页数：5

共 56 条

[1] Chalcones, coumarins, and flavanones from the exudate of Angelica keiskei and their chemopreventive effects [J].

Akihisa, T ;

Tokuda, H ;

Ukiya, M ;

Iizuka, M ;

Schneider, S ;

Ogasawara, K ;

Mukainaka, T ;

Iwatsuki, K ;

Suzuki, T ;

Nishino, H .

CANCER LETTERS, 2003, 201 (02) :133-137

[2] ANTICANCER AND ANTIOXIDANT ACTIVITY OF SYNTHETIC CHALCONES AND RELATED-COMPOUNDS [J].

ANTO, RJ ;

SUKUMARAN, K ;

KUTTAN, G ;

RAO, MNA ;

SUBBARAJU, V ;

KUTTAN, R .

CANCER LETTERS, 1995, 97 (01) :33-37

[3]

BALDI S, 2003, P 8 INT C MECH ANT A, P14

[4] Erythrocyte enzymes catalyze 1-nitropyrene and 3-nitrofluoranthene nitroreduction [J].

Belisario, MA ;

Pecce, R ;

Garofalo, A ;

Sannolo, N ;

Malorni, A .

TOXICOLOGY, 1996, 108 (1-2) :101-108

[5] Synthesis and biological evaluation of chalcones and their derived pyrazoles as potential cytotoxic agents [J].

Bhat, BA ;

Dhar, KL ;

Puri, SC ;

Saxena, AK ;

Shanmugavel, M ;

Qazi, GN .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2005, 15 (12) :3177-3180

[6]

BORS W, 1990, METHOD ENZYMOL, V186, P343

[7]

Brown JE, 1998, BIOCHEM J, V330, P1173

[8]

BURGER RM, 1979, J BIOL CHEM, V254, P906

[9] DNA STRAND BREAKAGE IN ISOLATED-NUCLEI SUBJECTED TO BLEOMYCIN OR HYDROGEN-PEROXIDE [J].

BYRNES, RW ;

PETERING, DH .

BIOCHEMICAL PHARMACOLOGY, 1994, 48 (03) :575-582

[10] Hydrogen peroxide regulation of endothelial function: Origins, mechanisms, and consequences [J].

Cai, H .

CARDIOVASCULAR RESEARCH, 2005, 68 (01) :26-36

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