In silico and experimental methods revealed highly diverse bacteria with quorum sensing and aromatics biodegradation systems - A potential broad application on bioremediation

被引：64

作者：

Huang, Yili ^{[1
]}

Zeng, Yanhua ^{[1
]}

Yu, Zhiliang ^{[2
]}

Zhang, Jing ^{[1
]}

Feng, Hao ^{[1
]}

Lin, Xiuchun ^{[3
]}

机构：

[1] Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Sci, Zhejiang Prov Key Lab Organ Pollut Proc & Control, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou, Zhejiang, Peoples R China

[3] Putian Univ, Dept Environm & Life Sci, Putian 351100, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 148卷

关键词：

Aromatic compounds; Biodegradation; In silica; Quorum sensing; Acyl homoserine lactone; HYDROCARBON-DEGRADING BACTERIA; LACTONE-PRODUCING BACTERIA; GENE; WATER; IDENTIFICATION; PLASMIDS;

D O I：

10.1016/j.biortech.2013.08.155

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Phylogenetic overlaps between aromatics-degrading bacteria and acyl-homoserine-lactone (AHL) or autoinducer (AI) based quorum-sensing (QS) bacteria were evident in literatures; however, the diversity of bacteria with both activities had never been finely described. In-silico searching in NCBI genome database revealed that more than 11% of investigated population harbored both aromatic ring-hydroxylating-dioxygenase (RHO) gene and AHL/AI-synthetase gene. These bacteria were distributed in 10 orders, 15 families, 42 genus and 78 species. Horizontal transfers of both genes were common among them. Using enrichment and culture dependent method, 6 Sphingomonadales and 4 Rhizobiales with phenanthrene- or pyrene-degrading ability and AHL-production were isolated from marine, wetland and soil samples. Thin-layer-chromatography and gas-chromatography-mass-spectrum revealed that these Sphingomonads produced various AHL molecules. This is the first report of highly diverse bacteria that harbored both aromatics-degrading and QS systems. QS regulation may have broad impacts on aromatics biodegradation, and would be a new angle for developing bioremediation technology. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：311 / 316

页数：6

共 24 条

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