Adequacy of Petrifilm™ Aerobic Count plates supplemented with de Man, Rogosa & Sharpe broth and chlorophenol red for enumeration of lactic acid bacteria in salami

被引：7

作者：

Augusto de Castilho, Natalia Parma ^{[1
]}

Okamura, Vivian Tiemi ^{[1
]}

Camargo, Anderson Carlos ^{[1
]}

Pieri, Fabio Alessandro ^{[1
,2
]}

Nero, Luis Augusto ^{[1
]}

机构：

[1] Univ Fed Vicosa, Dept Vet, BR-36570900 Vicosa, MG, Brazil

[2] Univ Fed Juiz de Fora, Dept Ciencias Basicas Saude, BR-35020220 Governador Valadares, MG, Brazil

来源：

MEAT SCIENCE | 2015年 / 110卷

关键词：

Petrifilm (TM) AC; Lactic acid bacteria; Salami; Enumeration; TYPICAL ITALIAN SALAMI; 16S RIBOSOMAL-RNA; STARTER CULTURE; STAPHYLOCOCCUS-XYLOSUS; MICROBIAL ECOLOGY; FLAVOR COMPOUNDS; IDENTIFICATION; PCR; BIFIDOBACTERIA;

D O I：

10.1016/j.meatsci.2015.07.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The present study aimed to assess the performance of alternative protocols to enumerate lactic acid bacteria (LAB) in salami. Fourteen cultures and two mixed starter cultures were plated using six protocols: 1) Petrifilm (TM) Aerobic Count (AC) with MRS broth and chlorophenol red (CR), incubated under aerobiosis or 2) under anaerobiosis, 3) MRS agar with CR, 4) MRS agar with bromocresol purple, 5) MRS agar at pH 5.7, and 6) All Purpose Tween agar. Samples of salami were obtained and the LAB microbiota was enumerated by plating according protocols 1, 2, 3 and 5. Regression analysis showed a significant correlation between the tested protocols, based on culture counts (p < 0.05). Similar results were observed for salami, and no significant differences of mean LAB counts between selected protocols (ANOVA, p > 0.05). Colonies were confirmed as LAB, indicating proper selectivity of the protocols. The results showed the adequacy of Petrifilm (TM) AC supplemented with CR for the enumeration of LAB in salami. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：253 / 261

页数：9

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