Salivary gland expression level of IκBα regulatory protein in Sjogren's syndrome

被引:12
作者
Sisto, Margherita [1 ]
Lisi, Sabrina [1 ]
Lofrumento, Dario Domenico [2 ]
Ingravallo, Giuseppe [3 ]
De Lucro, Raffaella [1 ]
D'Amore, Massimo [4 ]
机构
[1] Univ Bari, Dept Basic Med Sci Neurosci & Sense Organs, Sch Med, Lab Cell Biol,Sect Human Anat & Histol, I-70124 Bari, Italy
[2] Univ Salento, Dept Biol & Environm Sci & Technol, Lecce, Italy
[3] Univ Bari, Dept Pathol, Sch Med, I-70124 Bari, Italy
[4] Univ Bari, Dept Internal Med & Publ Med, Sch Med, Rheumatol Sect, I-70124 Bari, Italy
关键词
NF-kappa B; Sjogren's syndrome; I kappa B alpha; Inflammation; Autoimmunity; ANTI-LA AUTOANTIBODIES; CELL-LINE A-253; EPITHELIAL-CELLS; PROMOTER POLYMORPHISMS; RHEUMATOID-ARTHRITIS; ACTIVATION; GENE; APOPTOSIS; DISEASES; MICE;
D O I
10.1007/s10735-013-9487-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Diagnosis and therapeutic strategies in Sjogren's syndrome (SS) might greatly benefit of the present multidisciplinary approach to studying the molecular pathogenesis of the disease. A deregulated inflammatory response has been described in the SS. The research in the last years sheds light on the importance of the NF-kappa B pathway regulating the pro-inflammatory cytokine production and leukocyte recruitment. These are important contributors to the inflammatory response during the development of SS. In this study we examine the expression of the NF-kappa B inhibitory protein termed I kappa B alpha in salivary glands epithelial cells (SGEC) comparing it with SGEC from healthy controls, to test the hypothesis that an altered expression of I kappa B alpha occurs in SGEC from SS biopsies. Real-Time PCR, western blot and immunohistochemistry demonstrated that the expression level of I kappa B alpha was significantly lower in SS with respect to healthy controls leading to an increased NF-kappa B activity. Our results suggest that the analysis of I kappa B alpha expression at salivary gland epithelial cell level could be a potential new hallmark of SS progression and sustain a rationale to more deeply investigate the therapeutic potential of specific NF-kappa B inhibitors in SS.
引用
收藏
页码:447 / 454
页数:8
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