Lack of correlation between MYCN expression and the Warburg effect in neuroblastoma cell lines

被引:14
作者
Smith, Danielle J. [1 ,2 ]
Cossins, Luke R. [2 ]
Hatzinisiriou, Irene [2 ]
Haber, Michelle [3 ]
Nagley, Phillip [1 ,2 ]
机构
[1] Monash Univ, Australian Res Council, Ctr Excellence Struct & Funct Microbial Genom, Clayton, Vic 3800, Australia
[2] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
[3] Childrens Canc Inst Australia Med Res, Randwick, NSW 2031, Australia
基金
澳大利亚研究理事会; 英国医学研究理事会;
关键词
D O I
10.1186/1471-2407-8-259
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Many cancers preferentially meet their energy requirements through the glycolytic pathway rather than via the more efficient oxidative phosphorylation pathway. It is thought that this is an important adaptation in cancer malignancy. We investigated whether use of glycolysis for energy production even in the presence of oxygen (known as the Warburg effect) varied between neuroblastoma cell lines with or without MYCN amplification (a key indicator of poor disease outcome in neuroblastoma). Methods: We examined ATP and lactate production, oxygen consumption and mitochondrial energisation status for three neuroblastoma cell lines with varying degrees of MYCN amplification and MYCN expression. Results: We found no correlation between MYCN expression and the Warburg effect in the cell lines investigated. Conclusion: Our results suggest preferential use of glycolysis for energy production and MYCN expression may be independent markers of neuroblastoma malignancy in vitro if not in vivo.
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页数:8
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