Direct effects of transforming growth factor-β1 signaling on the differentiation fate of fetal hepatic progenitor cells

被引：0

作者：

Yasen, Aimaiti ^{[1
,2
]}

Li, Wending ^{[1
]}

Maimaitinijiati, Yusufukadier ^{[1
]}

Aini, Abudusalamu ^{[1
]}

Ran, Bo ^{[2
]}

Wang, Hui ^{[3
]}

Tuxun, Tuerhongjiang ^{[4
]}

Shao, Yingmei ^{[2
]}

Aji, Tuerganaili ^{[2
]}

Wen, Hao ^{[2
,5
]}

机构：

[1] Xinjiang Med Univ, Xinjiang Uyghur Autonomous Reg, Urumqi 830011, Peoples R China

[2] Xinjiang Med Univ, Digest & Vasc Surg Ctr, Dept Hepatobiliary & Hydatid Dis, Affiliated Hosp 1, Urumqi 830054, Peoples R China

[3] Xinjiang Med Univ, Clin Med Res Inst, Affiliated Hosp 1, Urumqi 830054, Xinjiang Uyghur, Peoples R China

[4] Xinjiang Med Univ, Digest & Vasc Surg Ctr, Dept Liver & Laparoscop Surg, Affiliated Hosp 1, Urumqi 830054, Xinjiang Uyghur, Peoples R China

[5] Xinjiang Med Univ, State Key Lab Pathogenesis Prevent & Treatment Hi, 393 Xin Yi Rd, Urumqi 830011, Xinjiang Uyghur, Peoples R China

来源：

REGENERATIVE MEDICINE | 2020年 / 15卷 / 06期

关键词：

development; differentiation; fetal hepatic progenitor cells; fetal liver; TGF-beta; 1; HEPATOCYTE TRANSPLANTATION; MOUSE-LIVER; EXPRESSION; HEPATOBLASTS; REPOPULATION; MATURATION; CULTURE; DEVELOP; JAGGED1; SERUM;

D O I：

10.2217/rme-2020-0002

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aim:To investigate direct roles of TGF-beta 1 signaling in the differentiation process of fetal hepatic progenitor cells (HPCs).Materials & methods:Exogenous TGF-beta 1 and SB431542 were added into fetal HPCs. Then, SB431542 was intraperitoneally injected into pregnant mice for 8 days.Results:Fetal HPCs treated with TGF-beta 1 differentiated into cholangiocytes. However, hepatocyte marker was highly expressed after inhibiting TGF-beta 1 signaling.In vivo, hematopoietic cells were gradually replaced with liver cells and TGF-beta 1 expression was evidently decreased as fetal liver developed. Inhibition of TGF-beta 1 signaling caused increase of ALB(+)cells, but CK19 expression was more obvious in control mice livers.Conclusion:TGF-beta 1 signaling may play decisive roles in fetal HPCs differentiation into functional hepatocytes or cholangiocytes.

引用

页码：1719 / 1733

页数：15