Study of the siderophore-producing Trichoderma asperellum Q1 on cucumber growth promotion under salt stress

Trichoderma spp. are versatile beneficial fungi which can stimulate growth and plant resistance to biotic and abiotic stresses. In this study, the potential of Trichoderma isolate in promoting the cucumber growth under salt stress and its possible mechanisms were investigated. Strain Q1 was isolated from the rhizosphere of cucumber in greenhouse in China and identified as Trichoderma asperellum based on its morphological features and the molecular phylogenetic analyses. It exhibited some plant growth-promoting attributes of phosphate solubilization, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, auxin and siderophore production. In pot trials, applying strain Q1 to cucumber plant had significantly promoted seedlings growth and alleviated the growth suppression induced by salt stress as confirmed by the changes in growth phenotype and several biochemical and physiological parameters. In solution culture experiments, the growth of cucumber seedlings was increased and the percentage of wilted cucumber seedlings was decreased in the treatment of siderophore-containing culture filtrate (SCF) of strain Q1 with insoluble Fe3+ under salt stress. These results indicated that T. asperellum Q1 has a real potential to enhance cucumber growth by inducing physiological protection under saline stress, and its siderophores showed sign of alleviating negative effect of salinity and available iron deficiency.