Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk

被引：18

作者：

Begovic, Jelena ^{[1
]}

Jovcic, Branko ^{[1
,2
]}

Papic-Obradovic, Milena ^{[3
]}

Veljovic, Katarina ^{[1
]}

Lukic, Jovanka ^{[1
]}

Kojic, Milan ^{[1
]}

Topisirovic, Ljubisa ^{[1
]}

机构：

[1] Univ Belgrade, Inst Mol Genet & Genet Engn, Lab Mol Genet Ind Microorganisms, Belgrade 11010, Serbia

[2] Univ Belgrade, Fac Biol, Belgrade 11000, Serbia

[3] Univ Belgrade, Sch Med, Gynecol & Obstet Clin, Belgrade 11010, Serbia

来源：

MICROBIOLOGICAL RESEARCH | 2013年 / 168卷 / 02期

关键词：

Human breast milk; S; epidermidis; Antibiotic susceptibility; Virulence; COAGULASE-NEGATIVE STAPHYLOCOCCI; RESISTANCE GENES; SEQUENCE; AUREUS; IDENTIFICATION; TRANSFORMATION; MICROBIOTA; MECHANISM; BACTERIA; STRAINS;

D O I：

10.1016/j.micres.2012.09.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential. (C) 2012 Elsevier GmbH. All rights reserved.

引用

页码：77 / 83

页数：7

共 48 条

[1] Molecular ecology of tetracycline resistance: Development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins [J].