Facile Synthesis of Kwakhurin, a Marker Compound of Pueraria mirifica and Its Quantitative NMR Analysis for Standardization as a Reagent

被引：5

作者：

Tsuji, Genichiro ^{[1
]}

Yusa, Masaaki ^{[1
]}

Masada, Sayaka ^{[1
]}

Yokoo, Hidetomo ^{[1
,2
]}

Hosoe, Junko ^{[1
]}

Hakamatsuka, Takashi ^{[1
]}

Demizu, Yosuke ^{[1
,2
]}

Uchiyama, Nahoko ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, 3-25-26 Tonomachi, Kawasaki, Kanagawa 2109501, Japan

[2] Yokohama City Univ, Grad Sch Med Life Sci, Tsurumi Ku, 1-7-29 Suehiro, Yokohama, Kanagawa 2300045, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2020年 / 68卷 / 08期

关键词：

kwakhurin; Pueraria mirifica; kwao keur; total synthesis; isoflavone; quantitative NMR; ENANTIOSELECTIVE TOTAL-SYNTHESIS; KWAO KEUR; PLANT;

D O I：

10.1248/cpb.c20-00346

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The side effects of kwao keur dietary supplements (obtained from the tuberous root of Pueraria mirifica) have recently been reported by the Ministry of Health, Labour and Welfare, Japan. To control the quality of kwao keur products, its ingredients need to be maintained by characteristic marker compounds, such as miroestrol, deoxymiroestrol, and kwakhurin (KWA). In this study, we described the facile synthesis of KWA, a marker compound of P. mirifica. Our revised synthetic method produced KWA with shorter steps and higher yield than the reported method. Furthermore, the absolute purity of KWA was determined by quantitative NMR analysis for standardization as a reagent, and its purity was 92.62 +/- 0.12%.

引用

页码：797 / 801

页数：5

共 3 条

[1] Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb "Kwao Keur' (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol
Shimokawa, Satoko
Kumamoto, Takuya
Ishikawa, Tsutomu
Takashi, Miho
Higuchi, Yoshihiro
Chaichantipyuth, Chaiyo
Chansakaow, Sunee
NATURAL PRODUCT RESEARCH, 2013, 27 (4-5) : 371 - 378
[2] Facile synthesis of deuterium-labeled denatonium cation and its application in the quantitative analysis of Bitrex by liquid chromatography-mass spectrometry
Bachor, Remigiusz
Kluczyk, Alicja
Stefanowicz, Piotr
Szewczuk, Zbigniew
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (21) : 6557 - 6561
[3] Facile synthesis of deuterium-labeled denatonium cation and its application in the quantitative analysis of Bitrex by liquid chromatography-mass spectrometry
Remigiusz Bąchor
Alicja Kluczyk
Piotr Stefanowicz
Zbigniew Szewczuk
Analytical and Bioanalytical Chemistry, 2015, 407 : 6557 - 6561

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