Down-regulation of cyclin B1 and up-regulation of Wee1 by berberine promotes entry of leukemia cells into the G2/M-phase of the cell cycle

被引:0
作者
Lin, CC
Lin, SY
Chung, JG
Lin, JP
Chen, GW
Kao, ST
机构
[1] China Med Univ, Sch Chinese Med, Taichung 404, Taiwan
[2] China Med Univ, Dept Microbiol, Taichung 404, Taiwan
[3] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan
[4] China Med Univ, Sch Biol Sci & Technol, Taichung 404, Taiwan
[5] Fong Yuan Hosp, Dept Hlth, Fong Yuan 420, Taiwan
[6] Hung Kuang Univ, Dept Food & Nutr, Shalu 433, Taiwan
关键词
berberine; leukemia HL-60; WEHI-3; G2/M arrest;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Berberine has a wide range of biological actions that suggest it may be of use in cancer prevention. It was previously reported that berberine induced cell cycle arrest, not only at the G0/G1-phase, but also at the G2/M-phase in a dose-dependent manner. However, the mechanism of berberine-induced G2/M-phase arrest in leukemia cells is not fully understood. In the present study, the effects of the naturally occurring berberine (the major constituent of Coptis chinensis) on the cell cycle, as well as on CDK1, cyclin B1, 14-3-3 sigma, Wee1 and Cdc25c expressions, were investigated in the human pronzyelocytic leukemia HL-60 cells and in the murine myelomonocytic leukemia WEHI-3 cells. The flow cytometry assays indicated that berberine induced G2/M-phase arrest in both examined cell lines. The berberine-induced G2/M-phase arrest in both examined cell lines was accompanied by increased levels of Wee1 and 14-3-3 sigma, but decreased levels of Cdc25c, CDK1 and cyclin B1 However, CDK2 expression was not affected as revealed by Western blotting assay. Berberine induced G2/M arrest in both the examined cells via the inhibition of cyclin B1 and the promotion of Wee1.
引用
收藏
页码:1097 / 1104
页数:8
相关论文
共 37 条
  • [1] Growth signaling in breast cancer cells: outcomes and promises of proteomics
    Adriaenssens, E
    Lemoine, J
    El Yazidi-Belkoura, I
    Hondermarck, H
    [J]. BIOCHEMICAL PHARMACOLOGY, 2002, 64 (5-6) : 797 - 803
  • [2] BERBERINE SULFATE - ANTIMICROBIAL ACTIVITY, BIOASSAY, AND MODE OF ACTION
    AMIN, AH
    SUBBAIAH, TV
    ABBASI, KM
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1969, 15 (09) : 1067 - &
  • [3] Requirement for p53 and p21 to sustain G2 arrest after DNA damage
    Bunz, F
    Dutriaux, A
    Lengauer, C
    Waldman, T
    Zhou, S
    Brown, JP
    Sedivy, JM
    Kinzler, KW
    Vogelstein, B
    [J]. SCIENCE, 1998, 282 (5393) : 1497 - 1501
  • [4] 14-3-3σ is required to prevent mitotic catastrophe after DNA damage
    Chan, TA
    Hermeking, H
    Lengauer, C
    Kinzler, KW
    Vogelstein, B
    [J]. NATURE, 1999, 401 (6753) : 616 - 620
  • [5] Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells
    Chen, HC
    Hsieh, WT
    Chang, WC
    Chung, JG
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 2004, 42 (08) : 1251 - 1257
  • [6] Peroxynitrite irreversibly inactivates the human xenobiotic-metabolizing enzyme arylamine N-acetyltransferase 1 (NAT1) in human breast cancer cells -: A cellular and mechanistic study
    Dairou, J
    Atmane, N
    Rodrigues-Lima, F
    Dupret, JM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (09) : 7708 - 7714
  • [7] Farhana L, 2002, CANCER RES, V62, P3842
  • [8] FREYA W, 2001, J NUTR, V131, P2197
  • [9] 14-3-3 proteins: Structure, function, and regulation
    Fu, HA
    Subramanian, RR
    Masters, SC
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2000, 40 : 617 - 647
  • [10] LEISHMANIA-DONOVANI - AMASTIGOTE INHIBITION AND MODE OF ACTIOR OF BERBERINE
    GHOSH, AK
    BHATTACHARYYA, FK
    GHOSH, DK
    [J]. EXPERIMENTAL PARASITOLOGY, 1985, 60 (03) : 404 - 413