Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus

被引：97

作者：

Abrams, Charles C. ^{[1
]}

Goatley, Lynnette ^{[1
]}

Fishbourne, Emma ^{[1
]}

Chapman, David ^{[1
]}

Cooke, Lyndsay ^{[1
]}

Oura, Christopher A. ^{[1
]}

Netherton, Christopher L. ^{[1
]}

Takamatsu, Haru-Hisa ^{[1
]}

Dixon, Linda K. ^{[1
]}

机构：

[1] Pirbright Inst, Woking GU24 0NF, Surrey, England

来源：

VIROLOGY | 2013年 / 443卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

African swine fever virus; Attenuation; Virulence; CATALYTIC SUBUNIT; E3L GENE; VACCINE; GENOME; IMMUNIZATION; REPLICATION; PERSISTENCE; DETERMINANT; MATURATION; SIMILARITY;

D O I：

10.1016/j.virol.2013.04.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains including OUR T88/1. Two genes, DP71L and DP96R were deleted from the OUR T88/3 genome to create recombinant virus OUR T88/3 Delta DP2. Deletion of these genes from virulent viruses has previously been shown to reduce ASFV virulence in domestic pigs. Groups of 6 pigs were immunised with deletion virus OUR T88/3 Delta DP2 or parental virus OUR T88/3 and challenged with virulent OUR T88/1 virus. Four pigs (66%) were protected by inoculation with the deletion virus OUR T88/3 Delta DP2 compared to 100% protection with the parental virus OUR T88/3. Thus the deletion of the two genes DP71L and DP96R from OUR T88/3 strain reduced its ability to protect pigs against challenge with virulent virus. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.

引用

页码：99 / 105

页数：7

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[1] Sequential deletion of genes from the African swine fever virus genome using the cre/loxP recombination system [J].