Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus

被引：95
作者：
Abrams, Charles C. [1 ]
Goatley, Lynnette [1 ]
Fishbourne, Emma [1 ]
Chapman, David [1 ]
Cooke, Lyndsay [1 ]
Oura, Christopher A. [1 ]
Netherton, Christopher L. [1 ]
Takamatsu, Haru-Hisa [1 ]
Dixon, Linda K. [1 ]
机构：
[1] Pirbright Inst, Woking GU24 0NF, Surrey, England
来源：
VIROLOGY | 2013年 / 443卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
African swine fever virus; Attenuation; Virulence; CATALYTIC SUBUNIT; E3L GENE; VACCINE; GENOME; IMMUNIZATION; REPLICATION; PERSISTENCE; DETERMINANT; MATURATION; SIMILARITY;
D O I：
10.1016/j.virol.2013.04.028
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains including OUR T88/1. Two genes, DP71L and DP96R were deleted from the OUR T88/3 genome to create recombinant virus OUR T88/3 Delta DP2. Deletion of these genes from virulent viruses has previously been shown to reduce ASFV virulence in domestic pigs. Groups of 6 pigs were immunised with deletion virus OUR T88/3 Delta DP2 or parental virus OUR T88/3 and challenged with virulent OUR T88/1 virus. Four pigs (66%) were protected by inoculation with the deletion virus OUR T88/3 Delta DP2 compared to 100% protection with the parental virus OUR T88/3. Thus the deletion of the two genes DP71L and DP96R from OUR T88/3 strain reduced its ability to protect pigs against challenge with virulent virus. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：99 / 105
页数：7
相关论文
共 22 条
  • [1] African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus
    O'Donnell, Vivian
    Holinka, Lauren G.
    Gladue, Douglas P.
    Sanford, Brenton
    Krug, Peter W.
    Lu, Xiqiang
    Arzt, Jonathan
    Reese, Bo
    Carrillo, Consuelo
    Risatti, Guillermo R.
    Borca, Manuel V.
    JOURNAL OF VIROLOGY, 2015, 89 (11) : 6048 - 6056
  • [2] Immunization of African Indigenous Pigs with Attenuated Genotype I African Swine Fever Virus OURT88/3 Induces Protection Against Challenge with Virulent Strains of Genotype I
    Mulumba-Mfumu, L. K.
    Goatley, L. C.
    Saegerman, C.
    Takamatsu, H. -H.
    Dixon, L. K.
    TRANSBOUNDARY AND EMERGING DISEASES, 2016, 63 (05) : E323 - E327
  • [3] Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain
    Ramirez-Medina, Elizabeth
    Vuono, Elizabeth
    O'Donnell, Vivian
    Holinka, Lauren G.
    Silva, Ediane
    Rai, Ayushi
    Pruitt, Sarah
    Carrillo, Consuelo
    Gladue, Douglas P.
    Borca, Manuel, V
    VIRUSES-BASEL, 2019, 11 (07):
  • [4] Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge
    O'Donnell, Vivian
    Risatti, Guillermo R.
    Holinka, Lauren G.
    Krug, Peter W.
    Carlson, Jolene
    Velazquez-Salinas, Lauro
    Azzinaro, Paul A.
    Gladue, Douglas P.
    Borca, Manuel V.
    JOURNAL OF VIROLOGY, 2017, 91 (01)
  • [5] Deletion of the H108R Gene Reduces Virulence of the Pandemic Eurasia Strain of African Swine Fever Virus with Surviving Animals Being Protected against Virulent Challenge
    Vuono, Elizabeth
    Ramirez-Medina, Elizabeth
    Silva, Ediane
    Rai, Ayushi
    Pruitt, Sarah
    Espinoza, Nallely
    Valladares, Alyssa
    Velazquez-Salinas, Lauro
    Gladue, Douglas P.
    Borca, Manuel V.
    JOURNAL OF VIROLOGY, 2022, 96 (14)
  • [6] Deletion of African swine fever virus interferon inhibitors from the genome of a virulent isolate reduces virulence in domestic pigs and induces a protective response
    Reis, Ana Luisa
    Abrams, Charles C.
    Goatley, Lynnette C.
    Netherton, Chris
    Chapman, Dave G.
    Sanchez-Cordon, Pedro
    Dixon, Linda K.
    VACCINE, 2016, 34 (39) : 4698 - 4705
  • [7] Deletion of African Swine Fever Virus Histone-like Protein, A104R from the Georgia Isolate Drastically Reduces Virus Virulence in Domestic Pigs
    Ramirez-Medina, Elizabeth
    Vuono, Elizabeth A.
    Pruitt, Sarah
    Rai, Ayushi
    Espinoza, Nallely
    Valladares, Alyssa
    Silva, Ediane
    Velazquez-Salinas, Lauro
    Borca, Manuel, V
    Gladue, Douglas P.
    VIRUSES-BASEL, 2022, 14 (05):
  • [8] Evaluation of the Deletion of African Swine Fever Virus E111R Gene from the Georgia Isolate in Virus Replication and Virulence in Domestic Pigs
    Ramirez-Medina, Elizabeth
    Velazquez-Salinas, Lauro
    Valladares, Alyssa
    Meyers, Amanda
    Burton, Leeanna
    Silva, Ediane
    Clark, Jason
    Borca, Manuel V.
    Gladue, Douglas P.
    VIRUSES-BASEL, 2024, 16 (09):
  • [9] Six adenoviral vectored African swine fever virus genes protect against fatal disease caused by genotype I challenge
    Portugal, Raquel
    Goldswain, Hannah
    Moore, Rebecca
    Tully, Matthew
    Harris, Katie
    Corla, Amanda
    Flannery, John
    Dixon, Linda K.
    Netherton, Christopher L.
    JOURNAL OF VIROLOGY, 2024, 98 (07)
  • [10] Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge
    Ramirez-Medina, Elizabeth
    Vuono, Elizabeth
    Rai, Ayushi
    Pruitt, Sarah
    Espinoza, Nallely
    Velazquez-Salinas, Lauro
    Pina-Pedrero, Sonia
    Zhu, James
    Rodriguez, Fernando
    Borca, Manuel, V
    Gladue, Douglas P.
    JOURNAL OF VIROLOGY, 2022, 96 (01)
123