Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury

被引：207

作者：

Kalani, Anuradha ^{[1
]}

Chaturvedi, Pankaj ^{[1
]}

Kamat, Pradip K. ^{[1
]}

Maldonado, Claudio ^{[1
,2
]}

Bauer, Philip ^{[2
]}

Joshuaa, Irving G. ^{[1
]}

Tyagi, Suresh C. ^{[1
]}

Tyagi, Neetu ^{[1
]}

机构：

[1] Univ Louisville, Sch Med, Dept Physiol, Louisville, KY 40292 USA

[2] Univ Louisville, Dept Surg, Louisville, KY 40292 USA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2016年 / 79卷

关键词：

Curcumin; Exosomes; Neurons; Stroke; CEREBRAL-ARTERY OCCLUSION; MESENCHYMAL STROMAL CELLS; BRAIN; ASTROCYTES; DISEASE; STROKE; NEURODEGENERATION; NEUROPROTECTION; MECHANISMS; MEDIATORS;

D O I：

10.1016/j.biocel.2016.09.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exo(cur)), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8-10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exo(cur) for 7 days. Similarly, two sham groups were made and one group received MESC-exo(cur) treatment. The study determined that MESC-exo(cur) treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exo(cur) treated-IR group showed reduced inflammation and N-methyl-D-aspartate receptor expression. Treatment of MESC-exo(cur) also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：360 / 369

页数：10

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[1] Bioavailability of curcumin: Problems and promises [J].