Molecular Approaches to Identify Helicobacter pylori Antimicrobial Resistance

被引：52

作者：

Megraud, Francis ^{[1
]}

Benejat, Lucie ^{[1
]}

Ngoyi, Esther Nina Ontsira ^{[1
]}

Lehours, Philippe ^{[1
]}

机构：

[1] Univ Bordeaux, INSERM, U853, Lab Bacteriol, F-33076 Bordeaux, France

来源：

GASTROENTEROLOGY CLINICS OF NORTH AMERICA | 2015年 / 44卷 / 03期

关键词：

Macrolides; Fluoroquinolones; Tetracyclines; Rifampins; Polymerase chain reaction; Fluorescent in situ hybridization; Real-time polymerase chain reaction; Dual priming oligonucleotide; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; 23S RIBOSOMAL-RNA; GASTRIC BIOPSY SPECIMENS; IN-SITU HYBRIDIZATION; MUTATIONS CONFERRING RESISTANCE; OLIGONUCLEOTIDE LIGATION ASSAY; ALLELE-SPECIFIC PCR; CLARITHROMYCIN RESISTANCE; POINT MUTATIONS;

D O I：

10.1016/j.gtc.2015.05.002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Antimicrobial susceptibility testing is needed to adapt Helicobacter pylori treatment to obtain the best results. Beside the standard phenotypic methods, molecular methods are increasingly used. The value of these molecular tests is that they are quick, independent of the transport conditions, easy to standardize, and commercial kits are available. In this article, these methods are reviewed, focusing on the determination of H pylori resistance to macrolides and fluoroquinolones, and mentioning also the methods used for tetracycline and rifampin.

引用

页码：577 / +

页数：21

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