A whole-genome radiation hybrid panel for goat

被引：11

作者：

Du, X. Y. ^{[1
]}

Womack, J. E. ^{[2
]}

Owens, K. E. ^{[2
]}

Elliott, J. S. ^{[2
]}

Sayre, B. ^{[3
]}

Bottcher, P. J. ^{[4
]}

Milan, D. ^{[5
]}

Podesta, M. Garcia ^{[6
]}

Zhao, S. H. ^{[1
]}

Malek, M. ^{[6
]}

机构：

[1] Huazhong Agr Univ, Dept Anim Sci & Technol, Wuhan 430070, Peoples R China

[2] Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA

[3] Virginia State Univ, Dept Biol, Petersburg, VA 23830 USA

[4] Food & Agr Org United Nations, Anim Prod & Hlth Div, I-00153 Rome, Italy

[5] INRA, Lab Genet Cellulaire & Biometrie & Intelligence A, FR INRA, F-31326 Castanet Tolosan, France

[6] IAEA, Dept Nucl Sci & Applicat, FAO IAEA Agr & Biotechnol Lab, A-1400 Vienna, Austria

来源：

SMALL RUMINANT RESEARCH | 2012年 / 105卷 / 1-3期

关键词：

Radiation hybrid panel; Goat; Gene mapping; CHRH5000; CHROMOSOME REARRANGEMENTS; RIVER BUFFALO; LINKAGE MAP; RH MAP; GENE; CATTLE; CONSTRUCTION; SHEEP;

D O I：

10.1016/j.smallrumres.2011.11.023

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A 5000 rad goat-hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and produced an average of 8.4 mg of DNA per radiation hybrid. A SINE-PCR test showed that almost all radiation hybrids retained goat DNA. The retention frequencies of the 121 hybrids were preliminarily estimated using a collection of 42 unlinked molecular markers. An optimized panel of therein 90 radiation hybrids (CHRH5000) with an average retention frequency of 34.2% was screened on the basis of interspersed repetitive DNA content and chromosome retention frequency. The development of this WG-RH panel will provide a fundamental tool for advanced goat genome mapping studies and for mammalian comparative mapping. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：114 / 116

页数：3

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