Multiplex PCRs for identification of Escherichia coli virulence genes

被引：143

作者：

Pass, MA

Odedra, R

Batt, RM

机构：

[1] Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia

[2] Univ London Royal Vet Coll, Dept Small Anim Med & Surg, Hatfield AL9 7TA, Herts, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 05期

关键词：

D O I：

10.1128/JCM.38.5.2001-2004.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

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页码：2001 / 2004

页数：4

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