Multiplex PCRs for identification of Escherichia coli virulence genes

被引：143
作者：
Pass, MA
Odedra, R
Batt, RM
机构：
[1] Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia
[2] Univ London Royal Vet Coll, Dept Small Anim Med & Surg, Hatfield AL9 7TA, Herts, England
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 05期
关键词：
D O I：
10.1128/JCM.38.5.2001-2004.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
引用
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页码：2001 / 2004
页数：4
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