EXPRESSION AND PURIFICATION OF PHYTOPHTHORA INFESTANS (MONT.) DE. BRAY RXLR EFFECTOR

The RXLR-class effectors play an important role in the pathogenesis of pathogens. They not only can enhance the pathogenicity of the pathogen, but also can be identified as a non-toxic protein to induce cell hypersensitive response (HR) by resistance protein of the host plant. In this study, the gene of RXLR effector was amplified by PCR, then the target gene and expression vector pET-28a were ligated and transformed into BL21( DE3) competent cells to obtain the pET-28a-RXLR recombinant expression plasmid. The protein was induced expressed by IPTG, purified by Ni affinity chromatography and detected by SDS-PAGE. The recombinant expression plasmid pET-28a-RXLR was successfully constructed and expressed in E. coli to produce a protein sized 18.4 kDa, consistent with the theoretical value. The high purity RXLR effector was successfully obtained in this study, laying a foundation for its pathogenicity study.