Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid

被引：195
作者：
Yeung, Man Lung [1 ]
Bennasser, Yamina [1 ]
Watashi, Koichi [1 ]
Le, Shu-Yun [2 ]
Houzet, Laurent [1 ]
Jeang, Kuan-Teh [1 ]
机构：
[1] NIAID, Mol Virol Sect, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA
[2] NCI, Ctr Canc Res, Nanobiol Program, Frederick, MD 21702 USA
来源：
NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 19期
关键词：
HUMAN-IMMUNODEFICIENCY-VIRUS; SMALL INTERFERING RNAS; MICRORNA EXPRESSION; REVERSE TRANSCRIPTION; TYPE-1; REPLICATION; CORE PROTEIN; HIV-1; DICER; SIRNA; SUPPRESSOR;
D O I：
10.1093/nar/gkp707
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Small non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 30 end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.
引用
收藏
页码：6575 / 6586
页数：12
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