Three-compartment electrodialysis device for on-line sample cleanup and enrichment prior to capillary electrophoresis

被引：27

作者：

Buscher, BAP ^{[1
]}

Tjaden, UR ^{[1
]}

vanderGreef, J ^{[1
]}

机构：

[1] LEIDEN UNIV,DIV ANALYT CHEM,LEIDEN AMSTERDAM CTR DRUG RES,NL-2300 RA LEIDEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 788卷 / 1-2期

关键词：

electrodialysis; instrumentation; sample handling; inositol phosphates;

D O I：

10.1016/S0021-9673(97)00707-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrodialysis device for on-line coupling to capillary electrophoresis has been developed. The device consists of three compartments that are separated by two membranes with M-r cut-off values of 500 and 30 000, respectively. The selectivity of the method is based on charge, molecular mass and shape. A concentration factor of 40-50 has been achieved. Sample clean-up and analyte enrichment take only ca. 5 min. Optimization and characterization of the device have been performed and electrodialysis has been applied to the analysis of an inositol trisphosphate derivative in a complex matrix. (C) 1997 Elsevier Science B.V.

引用

页码：165 / 172

页数：8

共 37 条

[1]

BENSON LM, 1994, HRC-J HIGH RES CHROM, V17, P671

[2] On-line electrodialysis capillary zone electrophoresis of adenosine triphosphate and inositol phosphates [J].

Buscher, BAP ;

Tjaden, UR ;

vanderGreef, J .

JOURNAL OF CHROMATOGRAPHY A, 1997, 764 (01) :135-142

[3] ANALYSIS OF INOSITOL PHOSPHATES AND DERIVATIVES USING CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY [J].

BUSCHER, BAP ;

VANDERHOEVEN, RAM ;

TJADEN, UR ;

ANDERSSON, E ;

VANDERGREEF, J .

JOURNAL OF CHROMATOGRAPHY A, 1995, 712 (01) :235-243

[4] DETERMINATION OF INOSITOL PHOSPHATES IN FERMENTATION BROTH USING CAPILLARY ZONE ELECTROPHORESIS WITH INDIRECT UV DETECTION [J].

BUSCHER, BAP ;

IRTH, H ;

ANDERSSON, E ;

TJADEN, UR ;

VANDERGREEF, J .

JOURNAL OF CHROMATOGRAPHY A, 1994, 678 (01) :145-150

[5] On-line electrodialysis capillary zone electrophoresis mass spectrometry of inositol phosphates in complex matrices [J].

Buscher, BAP ;

Hofte, AJP ;

Tjaden, UR ;

vanderGreef, J .

JOURNAL OF CHROMATOGRAPHY A, 1997, 777 (01) :51-60

[6] Z-SHAPED FLOW CELL FOR UV-DETECTION IN CAPILLARY ELECTROPHORESIS [J].

CHERVET, JP ;

VANSOEST, REJ ;

URSEM, M .

JOURNAL OF CHROMATOGRAPHY, 1991, 543 (02) :439-449

[7]

Chien R. L., 1992, ANAL CHEM, V64, P489

[8] SAMPLE STACKING OF AN EXTREMELY LARGE INJECTION VOLUME IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].

CHIEN, RL ;

BURGI, DS .

ANALYTICAL CHEMISTRY, 1992, 64 (09) :1046-1050

[9] QUANTITATIVE ENRICHMENT OF TRACE LEVELS OF IONS BY ELECTRODIALYSIS [J].

COX, JA ;

CARLSON, R .

ANALYTICA CHIMICA ACTA, 1981, 130 (02) :313-321

[10] SWITCHING VALVE WITH INTERNAL MICRO PRECOLUMN FOR ONLINE SAMPLE ENRICHMENT IN CAPILLARY ZONE ELECTROPHORESIS [J].

DEBETS, AJJ ;

MAZEREEUW, M ;

VOOGT, WH ;

VANIPEREN, DJ ;

LINGEMAN, H ;

HUPE, KP ;

BRINKMAN, UAT .

JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :151-158

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