Proteomic Analysis of the Oxidative Stress Response Induced by Low-Dose Hydrogen Peroxide in Bacillus anthracis

被引:16
作者
Kim, Sang Hoon [1 ]
Kim, Se Kye [1 ]
Jung, Kyoung Hwa [1 ]
Kim, Yun Xi [2 ]
Hwang, Hyun Chul [2 ]
Ryu, Sam Gon [3 ]
Chai, Young Gyu [1 ]
机构
[1] Hanyang Univ, Dept Mol & Life Sci, Ansan 426791, South Korea
[2] Samyang Chem Co Ltd, Anyang 430852, South Korea
[3] Agcy Def Dev, Taejon 305152, South Korea
关键词
Bacillus anthracis; hydrogen peroxide; oxidative stress; proteomics; DIHYDRODIPICOLINATE SYNTHASE; PROTEIN EXPRESSION; SUPEROXIDE; SUBTILIS; SPORES; RESISTANCE; VIRULENCE;
D O I
10.4014/jmb.1209.09070
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Anthrax is a bacterial disease caused by the aerobic spore-forming bacterium Bacillus anthracis, which is an important pathogen owing to its ability to be used as a terror agent. B. anthracis spores can escape phagocytosis and initiate the germination process even in antimicrobial conditions, such as oxidative stress. To analyze the oxidative stress response in B. anthracis and thereby learn how to prevent antimicrobial resistance, we performed protein expression profiling of B. anthracis strain HY1 treated with 0.3 mM hydrogen peroxide using a comparative proteomics-based approach. The results showed a total of 60 differentially expressed proteins; among them, 17 showed differences in expression over time. We observed time-dependent changes in the production of metabolic and repair/protection signaling proteins. These results will be useful for uncovering the metabolic pathways and protection mechanisms of the oxidative response in B. anthracis.
引用
收藏
页码:750 / 758
页数:9
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