Electrochemical Behavior and Determination of Matrine by the Reduced Graphene Oxide-Nafion Modified Glassy Carbon Electrode

Reduced graphene oxide-Nafion composite modified glassy carbon electrode (RGO-Nafion/GCE) was prepared and the electrochemical behavior of matrine at RGO-Nafion/GCE was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). An irreversible oxidation peak of matrine was observed in 0.2 mol . L-1 Na2HPO4-NaH2PO4 buffer system (pH 7.2) with 1.05 V of E-pa (vs. SCE). A novel method for the determination of matrine was developed based on a well-defined electrochemical response signal of matrine at RGO-Nafion/GCE. Under the optimized conditions, the DPV oxidation peak current and the concentration of matrine showed a good linear relationship in the range from 5.0x10(-5) to 3.0x10(-3) mol . L-1, the linear regression equation was I-pa (mu A) = 7.145c+0.3778 (r=0.9990) and the detection limit for matrine could reach 2.5x10(-6) mol . L-1. The recovery of matrine was 99.90-101.82% (RSD=1.3%) in huam serum. The proposed method is quick, sensitive, reliable, and can be used for the determination of matrine.