APPLICATION OF RAMAN SPECTROSCOPY FOR ANALYSIS OF CARBON NANOTUBE DISTRIBUTION IN LIVING CELLS

被引：8

作者：

Golubewa, E. N. ^{[1
]}

Shuba, M. V. ^{[2
]}

Vasilieu, M. V. ^{[1
]}

Kulahava, T. A. ^{[1
]}

机构：

[1] Belorussian State Univ, 4 Nezavisimost Ave, Minsk 220030, BELARUS

[2] Belorussian State Univ, Res Inst Nucl Problems, Minsk, BELARUS

来源：

JOURNAL OF APPLIED SPECTROSCOPY | 2019年 / 85卷 / 06期

关键词：

carbon nanotubes; Raman spectroscopy; glioma cells; local concentration; intracellular distribution; MICE;

D O I：

10.1007/s10812-019-00768-7

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We have used Raman spectroscopy combined with confocal microscopy to study suspensions of single-wall and double-wall carbon nanotubes of different lengths and also multiwall carbon nanotubes. We have shown that the intensity of the G mode in the Raman spectrum of carbon nanotubes is directly proportional to the nanotube concentration, the exposure time, the exciting radiation power, and depth of focus in the transparent sample under study. We have established that the Raman spectra of longer carbon nanotubes (similar to 1 mu m) are characterized by higher intensity of the G mode compared with short carbon nanotubes (similar to 250-450 nm). The dependences obtained were used to determine the local intracellular concentration of carbon nanotubes within the waist of the exciting laser beam, with the aim of mapping the carbon nanotube distribution inside the cells.

引用

页码：1121 / 1127

页数：7

共 22 条

[1]

[Anonymous], 2012, J NANOMEDICINE NANOT, DOI [10.4172/2157-7439.1000140, DOI 10.4172/2157-7439.1000140]

[2] Single walled carbon nanotube quantification method employing the Raman signal intensity [J].

Anoshkin, Ilya V. ;

Nefedova, Irina I. ;

Lioubtchenko, Dmitri V. ;

Nefedov, Igor S. ;

Raisanen, Antti V. .

CARBON, 2017, 116 :547-552

[3] Spectroscopic characterization of protein-wrapped single-wall carbon nanotubes and quantification of their cellular uptake in multiple cell generations [J].

Bertulli, Cristina ;

Beeson, Harry J. ;

Hasan, Tawfique ;

Huang, Yan Yan S. .

NANOTECHNOLOGY, 2013, 24 (26)

[4] In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues [J].

Biris, Alexandru S. ;

Galanzha, Ekaterina I. ;

Li, Zhongrui ;

Mahmood, Meena ;

Xu, Yang ;

Zharov, Vladimir P. .

JOURNAL OF BIOMEDICAL OPTICS, 2009, 14 (02)

[5] Carbon Nanotubes in Cancer Therapy and Drug Delivery [J].

Elhissi, Abdelbary M. A. ;

Ahmed, Waqar ;

Hassan, Israr Ul ;

Dhanak, Vinod. R. ;

D'Emanuele, Antony .

JOURNAL OF DRUG DELIVERY, 2012, 2012

[6] Optical transition energies for carbon nanotubes from resonant Raman spectroscopy: Environment and temperature effects [J].

Fantini, C ;

Jorio, A ;

Souza, M ;

Strano, MS ;

Dresselhaus, MS ;

Pimenta, MA .

PHYSICAL REVIEW LETTERS, 2004, 93 (14) :147406-1

[7] Single-walled carbon nanotube spectroscopy in live cells: Towards long-term labels and optical sensors [J].

Heller, DA ;

Baik, S ;

Eurell, TE ;

Strano, MS .

ADVANCED MATERIALS, 2005, 17 (23) :2793-+

[8] Quantification of Uptake and Localization of Bovine Serum Albumin-Stabilized Single-Wall Carbon Nanotubes in Different Human Cell Types [J].

Holt, Brian D. ;

Dahl, Kris Noel ;

Islam, Mohammad F. .

SMALL, 2011, 7 (16) :2348-2355

[9] Raman intensity measurements of single-walled carbon nanotube suspensions as a quantitative technique to assess purity [J].

Irurzun, Veronica M. ;

Ruiz, M. Pilar ;

Resasco, Daniel E. .

CARBON, 2010, 48 (10) :2873-2881

[10] Measuring Uptake Dynamics of Multiple Identifiable Carbon Nanotube Species via High-Speed Confocal Raman Imaging of Live Cells [J].

Kang, Jeon Woong ;

Nguyen, Freddy T. ;

Lue, Niyom ;

Dasari, Ramachandra R. ;

Heller, Daniel A. .

NANO LETTERS, 2012, 12 (12) :6170-6174

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