A toddler SHIME® model to study microbiota of young children

被引：16

作者：

Bondue, Pauline ^{[1
]}

Lebrun, Sarah ^{[1
]}

Taminiau, Bernard ^{[1
]}

Everaert, Nadia ^{[2
]}

LaPointe, Gisele ^{[3
]}

Crevecoeur, Sebastien ^{[1
]}

Daube, Georges ^{[1
]}

Delcenserie, Veronique ^{[1
]}

机构：

[1] Univ Liege, Dept Food Sci, Fundamental & Appl Res Anim & Hlth, Fac Vet Med, Quartier Vallee 2,Ave Cureghem 10, B-4000 Liege, Belgium

[2] Univ Liege, Gembloux Agrobio Tech, Precis Livestock & Nutr Unit, Passage Deportes 2, B-5030 Gembloux, Belgium

[3] Univ Guelph, Canadian Res Inst Food Safety, Guelph, ON N1G 2W1, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 2020年 / 367卷 / 16期

关键词：

SHIME (R) model; microbiota; toddler; biofilm; Megasphera massiliensis; Enterobacter cloacae; DYNAMIC GUT MODEL; IN-VITRO MODEL; COLONIC FERMENTATION; COMMUNITY; SIMULATOR; HEALTH; FOOD;

D O I：

10.1093/femsle/fnaa135

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 'first 1000 days of life' determine the gut microbiota composition and can have long-term health consequences. In this study, the simulator of the human intestinal microbial ecosystem (SHIME (R)) model, which represents the main functional sections of the digestive tract, was chosen to study the microbiota of young children. The aim of this study was to reproduce the digestive process of toddlers and their specific colonic environment. The ascending, transverse and descending colons of SHIME (R) model were inoculated with feces from three donors aged between 1 and 2 years-old, in three separate runs. For each run, samples from colon vessels were collected at days 14, 21 and 28 after microbiota stabilization period. Short chain fatty acid concentrations determined by HPLC showed that microbiota obtained in SHIME (R) model shared characteristics between adults and infants. In addition, microbial diversity and bacterial populations determined by 16S rRNA amplicon sequencing were specific to each colon vessel. In conclusion, the SHIME (R) model developed in this study seemed well adapted to evaluate prebiotic and probiotic impact on the specific microbiota of toddlers, or medicine and endocrine disruptor metabolism. Moreover, this study is the first to highlight some biofilm development in in vitro gastrointestinal modelling systems.

引用

页数：8

共 34 条

[1]

Asati S, 2017, Ann Burns Fire Disasters, V30, P39

[2] Immobilization of infant fecal microbiota and utilization in an in vitro colonic fermentation model [J].