Arsenite Tolerance is Related to Proportional Thiolic Metabolite Synthesis in Rice (Oryza sativa L.)

被引:46
|
作者
Dave, Richa [1 ,2 ]
Singh, Pradyumna Kumar [1 ]
Tripathi, Preeti [1 ]
Shri, Manju [1 ]
Dixit, Garima [1 ]
Dwivedi, Sanjay [1 ]
Chakrabarty, Debasis [1 ]
Trivedi, Prabodh Kumar [1 ]
Sharma, Yogesh Kumar [3 ]
Dhankher, Om Prakash [4 ]
Javier Corpas, Francisco [5 ]
Barroso, Juan B. [6 ]
Tripathi, Rudra Deo [1 ]
机构
[1] Natl Bot Res Inst, Council Sci & Ind Res, Lucknow 226001, Uttar Pradesh, India
[2] Amity Univ, Amity Inst Environm Sci, Noida 201303, India
[3] Univ Lucknow, Dept Bot, Lucknow 226002, Uttar Pradesh, India
[4] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA
[5] CSIC, Dept Bioquim Biol Celular & Mol Plantas, EEZ, E-18080 Granada, Spain
[6] Univ Jaen, Unidad Asociada, EEZ, Area Bioquim & Biol Mol, Jaen 23071, Spain
关键词
PHYTOCHELATIN COMPLEXES; OXIDATIVE STRESS; ARSENATE; ACCUMULATION; TRANSLOCATION; RESPONSES;
D O I
10.1007/s00244-012-9818-8
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Thiol metabolism is the primary detoxification strategy by which rice plants tolerate arsenic (As) stress. In light of this, it is important to understand the importance of harmonised thiol metabolism with As accumulation and tolerance in rice plant. For this aim, tolerant (T) and sensitive (S) genotypes were screened from 303 rice (Oryza sativa) genotypes on exposure to 10 and 25 mu M arsenite (As-III) in hydroponic culture. On further As accumulation estimation, contrasting (13-fold difference) T (IC-340072) and S (IC-115730) genotypes were selected. This difference was further evaluated using biochemical and molecular approaches to understand involvement of thiolic metabolism vis-a-vis As accumulation in these two genotypes. Various phytochelatin (PC) species (PC2, PC3 and PC4) were detected in both the genotypes with a dominance of PC3. However, PC concentrations were greater in the S genotype, and it was noticed that the total PC (PC2 + PC3 + PC4)-to-As-III molar ratio (PC-SH:As-III) was greater in T (2.35 and 1.36 in shoots and roots, respectively) than in the S genotype (0.90 and 0.15 in shoots and roots, respectively). Expression analysis of several metal(loid) stress-related genes showed significant upregulation of glutaredoxin, sulphate transporter, and ascorbate peroxidase in the S genotype. Furthermore, enzyme activity of phytochelatin synthase and cysteine synthase was greater on As accumulation in the S compared with the T genotype. It was concluded that the T genotype synthesizes adequate thiols to detoxify metalloid load, whereas the S genotype synthesizes greater but inadequate levels of thiols to tolerate an exceedingly greater load of metalloids, as evidenced by thiol-to-metalloid molar ratios, and therefore shows a phytotoxicity response.
引用
收藏
页码:235 / 242
页数:8
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