Arsenite Tolerance is Related to Proportional Thiolic Metabolite Synthesis in Rice (Oryza sativa L.)

被引:46
|
作者
Dave, Richa [1 ,2 ]
Singh, Pradyumna Kumar [1 ]
Tripathi, Preeti [1 ]
Shri, Manju [1 ]
Dixit, Garima [1 ]
Dwivedi, Sanjay [1 ]
Chakrabarty, Debasis [1 ]
Trivedi, Prabodh Kumar [1 ]
Sharma, Yogesh Kumar [3 ]
Dhankher, Om Prakash [4 ]
Javier Corpas, Francisco [5 ]
Barroso, Juan B. [6 ]
Tripathi, Rudra Deo [1 ]
机构
[1] Natl Bot Res Inst, Council Sci & Ind Res, Lucknow 226001, Uttar Pradesh, India
[2] Amity Univ, Amity Inst Environm Sci, Noida 201303, India
[3] Univ Lucknow, Dept Bot, Lucknow 226002, Uttar Pradesh, India
[4] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA
[5] CSIC, Dept Bioquim Biol Celular & Mol Plantas, EEZ, E-18080 Granada, Spain
[6] Univ Jaen, Unidad Asociada, EEZ, Area Bioquim & Biol Mol, Jaen 23071, Spain
关键词
PHYTOCHELATIN COMPLEXES; OXIDATIVE STRESS; ARSENATE; ACCUMULATION; TRANSLOCATION; RESPONSES;
D O I
10.1007/s00244-012-9818-8
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Thiol metabolism is the primary detoxification strategy by which rice plants tolerate arsenic (As) stress. In light of this, it is important to understand the importance of harmonised thiol metabolism with As accumulation and tolerance in rice plant. For this aim, tolerant (T) and sensitive (S) genotypes were screened from 303 rice (Oryza sativa) genotypes on exposure to 10 and 25 mu M arsenite (As-III) in hydroponic culture. On further As accumulation estimation, contrasting (13-fold difference) T (IC-340072) and S (IC-115730) genotypes were selected. This difference was further evaluated using biochemical and molecular approaches to understand involvement of thiolic metabolism vis-a-vis As accumulation in these two genotypes. Various phytochelatin (PC) species (PC2, PC3 and PC4) were detected in both the genotypes with a dominance of PC3. However, PC concentrations were greater in the S genotype, and it was noticed that the total PC (PC2 + PC3 + PC4)-to-As-III molar ratio (PC-SH:As-III) was greater in T (2.35 and 1.36 in shoots and roots, respectively) than in the S genotype (0.90 and 0.15 in shoots and roots, respectively). Expression analysis of several metal(loid) stress-related genes showed significant upregulation of glutaredoxin, sulphate transporter, and ascorbate peroxidase in the S genotype. Furthermore, enzyme activity of phytochelatin synthase and cysteine synthase was greater on As accumulation in the S compared with the T genotype. It was concluded that the T genotype synthesizes adequate thiols to detoxify metalloid load, whereas the S genotype synthesizes greater but inadequate levels of thiols to tolerate an exceedingly greater load of metalloids, as evidenced by thiol-to-metalloid molar ratios, and therefore shows a phytotoxicity response.
引用
收藏
页码:235 / 242
页数:8
相关论文
共 50 条
  • [21] Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.)
    Wu, Lin-Bo
    Ueda, Yoshiaki
    Lai, Shang-Kun
    Frei, Michael
    PLANT CELL AND ENVIRONMENT, 2017, 40 (04): : 570 - 584
  • [22] Relative toxicity of arsenite and arsenate on germination and early seedling growth of rice (Oryza sativa L.)
    Md. Joinal Abedin
    Andy A. Meharg
    Plant and Soil, 2002, 243 : 57 - 66
  • [23] Relative toxicity of arsenite and arsenate on germination and early seedling growth of rice (Oryza sativa L.)
    Abedin, MJ
    Meharg, AA
    PLANT AND SOIL, 2002, 243 (01) : 57 - 66
  • [24] A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)
    Singh, Amit Pal
    Dixit, Garima
    Kumar, Amit
    Mishra, Seema
    Kumar, Navin
    Dixit, Sameer
    Singh, Pradyumna Kumar
    Dwivedi, Sanjay
    Trivedi, Prabodh Kumar
    Pandey, Vivek
    Dhankher, Om Prakash
    Norton, Gareth J.
    Chakrabarty, Debasis
    Tripathi, Rudra Deo
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2017, 115 : 163 - 173
  • [25] Cultivar differences in photosynthetic tolerance to photooxidation and shading in rice (Oryza sativa L.)
    Jiao, DM
    Li, X
    PHOTOSYNTHETICA, 2001, 39 (02) : 167 - 175
  • [26] Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.)
    Verma, S. K.
    Xalxo, M. S.
    Saxena, R. R.
    Verulkar, S. B.
    INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 2014, 74 (01) : 86 - 89
  • [27] Ammonium enhances the tolerance of rice seedlings (Oryza sativa L.) to drought condition
    Li, Yong
    Gao, Yingxu
    Ding, Lei
    Shen, Qirong
    Guo, Shiwei
    AGRICULTURAL WATER MANAGEMENT, 2009, 96 (12) : 1746 - 1750
  • [28] The role of root system traits in the drought tolerance of rice (Oryza sativa L.)
    Abd Allah, A.A.
    Badawy, Shimaa A
    Zayed, B.A.
    El Gohary, A.A.
    World Academy of Science, Engineering and Technology, 2011, 80 : 700 - 704
  • [29] Effects of abscisic acid on ozone tolerance of rice (Oryza sativa L.) seedlings
    Lin, DI
    Lur, HS
    Chu, C
    PLANT GROWTH REGULATION, 2001, 35 (03) : 295 - 300
  • [30] A critical review on the improvement of drought stress tolerance in rice (Oryza sativa L.)
    Rasheed, Adnan
    Hassan, Muhammad U.
    Aamer, Muhammad
    Batool, Maria
    Fang, Sheng
    Wu, Ziming
    Liu, Huijie
    NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, 2020, 48 (04) : 1756 - 1788