Genetic Diversity among the Species of Michelia in China using ISSR

Michelia as an evergreen tree or shrub contains 80 species in the world out of which nearly 70 species could be found in China. In this study, Inter-simple Sequence Repeat Amplification was used to analyze the genetic diversity of 48 species of Michelia. A total of 151 loci were detected in 10 primers, and all were polymorphic, showing abundant polymorphism. According to the analysis of PopGen32 software package, for these 48 varieties, the Nei's gene diversity index (H), Shannon diversity index (I), coefficient gene differentiation (Gst) and gene flow (Nm) were 0.3261, 0.4946, 0.4838 and 0.5336, respectively. There was extensive genetic diversity among them. UPGMA clustering method divided these 48 species into seven categories, the clustering results support Nooteboom and Chen (2000) system's classification of Michelia. We suggest preserving the M. sphaerantha in the allotrope group. (C) 2020 Friends Science Publishers