Corynebacterium aquatimens sp nov., a lipophilic Corynebacterium isolated from blood cultures of a patient with bacteremia

被引：14

作者：

Aravena-Roman, M. ^{[2
]}

Sproeer, C. ^{[3
]}

Siering, C. ^{[4
]}

Inglis, T. ^{[2
]}

Schumann, P. ^{[3
]}

Yassin, A. F. ^{[1
]}

机构：

[1] Univ Bonn, Inst Med Mikrobiol & Immunol, D-53127 Bonn, Germany

[2] QEII MC, Lab Med Western Australia PathW, Div Microbiol & Infect Dis, Nedlands, WA 6909, Australia

[3] Leibniz Inst DSMZ Deutsch Sammlung Mikroorganisme, D-38124 Braunschweig, Germany

[4] Johannes Gutenberg Univ Mainz, Inst Organ Chem, D-55128 Mainz, Germany

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2012年 / 35卷 / 06期

关键词：

Corynebacterium aquatimens sp nov; Mycolic acid; CFA; 165 rRNA gene; rpoB gene; DNA-DNA hybridization; RAPD-PCR; MALDI-TOF MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; ANTIMICROBIAL SUSCEPTIBILITIES; CORYNEFORM BACTERIA; RENATURATION RATES; DNA HYBRIDIZATION; INFECTIONS; TREES; ACID;

D O I：

10.1016/j.syapm.2012.06.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An unknown lipophilic coryneform bacterium isolated from the blood cultures of a patient with bacteremia was characterized by phenotypic and molecular genetic methods. Chemical analysis revealed the presence of short chain mycolic acids consistent with the genus Corynebacterium. The DNA G+C content was 60.8 mol%. Comparative 165 rRNA gene sequence analysis demonstrated that the isolate represents a new subline within the genus Corynebacterium. The closely phylogenetic relative of the unknown bacterium was found to be C. tuscaniense (97.8% sequence similarity). Partial rpoB gene sequence revealed that strain IMMIB L-2475(T) exhibited 13.5% sequence divergence with C. tuscaniense. The unknown bacterium was distinguished from C. tuscaniense by, DNA-DNA hybridization, cellular fatty acid profiles, MALDI-TOF analyses of cell extracts and biochemical tests. Based on the phylogenetic and phenotypic criteria, it is proposed that this bacterium be classified as new species. Corynebacterium aquatimens sp. nov., and is represented by strain IMMIB L-2475(T) (=DSM 45632(T) = CCUG 61574(T)). (c) 2012 Elsevier GmbH. All rights reserved.

引用

页码：380 / 384

页数：5

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