Microwave-assisted synthesis of BSA-stabilized and HSA-protected gold nanoclusters with red emission

被引:149
作者
Yan, Lei [1 ]
Cai, Yuqing [1 ]
Zheng, Baozhan [1 ]
Yuan, Hongyan [2 ]
Guo, Yong [1 ]
Xiao, Dan [1 ,2 ]
Choi, Martin M. F. [3 ]
机构
[1] Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China
[2] Sichuan Univ, Coll Chem Engn, Chengdu 610064, Peoples R China
[3] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
ORGANIC-SYNTHESIS; NANOPARTICLES; FLUORESCENT; DNA; ATTACHMENT; BIOSENSOR; ELECTRODE; GLUCOSE; ROUTE; OVENS;
D O I
10.1039/c1jm13457d
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A microwave (MW)-assisted synthesis method for preparation of fluorescent BSA-stabilized and HSA-protected gold nanoclusters (BSA-AuNCs and HSA-AuNCs) has been developed. The reaction time can be shortened from tens of hours to several minutes, thanks to the superheating and non-thermal effects of the MW energy. The optimal experimental conditions including concentrations of BSA, HAuCl4 and NaOH and the MW programme are investigated in detail. The as-synthesised BSA-AuNC product is well characterized by UV-vis spectroscopy, fluorescence spectroscopy, HRTEM, XPS, and IR spectroscopy. The HSA-AuNCs display strong red emission which is efficiently quenched by nitrogen oxides (NOx), demonstrating its great potential in determining the intracellular concentration of NOx. The proposed MW-assisted synthesis method is simple, fast and should be applicable to prepare various protein-or enzyme-protected metal nanoclusters.
引用
收藏
页码:1000 / 1005
页数:6
相关论文
共 37 条
[1]   Organization of 'nanocrystal molecules' using DNA [J].
Alivisatos, AP ;
Johnsson, KP ;
Peng, XG ;
Wilson, TE ;
Loweth, CJ ;
Bruchez, MP ;
Schultz, PG .
NATURE, 1996, 382 (6592) :609-611
[2]   Nanoparticle-free synthesis of fluorescent gold nanoclusters at physiological temperature [J].
Bao, Yuping ;
Zhong, Chang ;
Vu, Dung M. ;
Temirov, Jamshid P. ;
Dyer, R. Brian ;
Martinez, Jennifer S. .
JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (33) :12194-12198
[3]   REACTIONS OF H-ATOMS PRODUCED BY MICROWAVE-DISCHARGE WITH OLEFINS IN ACETONE AND TOLUENE [J].
BEERI, A ;
BERMAN, E ;
VISHKAUTSAN, R ;
MAZUR, Y .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (20) :6413-6414
[4]   Microwave-assisted synthesis of luminescent LaPO4:Ce,Tb nanocrystals in ionic liquids [J].
Buehler, Gunnar ;
Feldmann, Claus .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (29) :4864-4867
[5]   CRYSTALLOGRAPHIC STRUCTURE OF SMALL GOLD PARTICLES STUDIED BY HIGH-RESOLUTION ELECTRON-MICROSCOPY [J].
BUFFAT, PA ;
FLUELI, M ;
SPYCHER, R ;
STADELMANN, P ;
BOREL, JP .
FARADAY DISCUSSIONS, 1991, 92 :173-187
[6]   On the Synthesis of Carbon Nanofibers and Nanotubes by Microwave Irradiation: Parameters, Catalysts, and Substrates [J].
Druzhinina, Tamara ;
Hoeppener, Stephanie ;
Schubert, Ulrich S. .
ADVANCED FUNCTIONAL MATERIALS, 2009, 19 (17) :2819-2825
[7]   Etching colloidal gold nanocrystals with hyperbranched and multivalent polymers: A new route to fluorescent and water-soluble atomic clusters [J].
Duan, Hongwei ;
Nie, Shuming .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (09) :2412-+
[8]   THE USE OF MICROWAVE-OVENS FOR RAPID ORGANIC-SYNTHESIS [J].
GEDYE, R ;
SMITH, F ;
WESTAWAY, K ;
ALI, H ;
BALDISERA, L ;
LABERGE, L ;
ROUSELL, J .
TETRAHEDRON LETTERS, 1986, 27 (03) :279-282
[9]   Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling [J].
Gelamo, EL ;
Silva, CHTP ;
Imasato, H ;
Tabak, M .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :84-99
[10]   APPLICATION OF COMMERCIAL MICROWAVE-OVENS TO ORGANIC-SYNTHESIS [J].
GIGUERE, RJ ;
BRAY, TL ;
DUNCAN, SM ;
MAJETICH, G .
TETRAHEDRON LETTERS, 1986, 27 (41) :4945-4948