Short, terminally modified 2′-OMe RNAs as inhibitors of microRNA

被引：5

作者：

Blechinger, Jenny ^{[1
]}

Pieper, Hanna ^{[1
]}

Marzenell, Paul ^{[1
]}

Kovbasyuk, Larisa ^{[1
]}

Serva, Andrius ^{[2
]}

Starkuviene, Vytaute ^{[2
]}

Erfle, Holger ^{[2
]}

Mokhir, Andriy ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, D-91054 Erlangen, Germany

[2] Heidelberg Univ, BioQuant, Heidelberg, Germany

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 67期

关键词：

ANTISENSE OLIGONUCLEOTIDES; DOWN-REGULATION; DNA; MICE; DEGRADATION; DERIVATIVES; COMMITMENT; PNA;

D O I：

10.1039/c3cc43174f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We applied 14-mer 2'-OMe RNAs as inhibitors of selected micro RNAs. To improve their properties, we introduced a trimethoxystilbene residue at the 5'-terminus and three 2'-fluoro-2'-deoxynucleotides at the 3'-terminus to obtain potent inhibitors, whose mismatch discrimination is substantially better than that of typically applied >18-mers.

引用

收藏

页码：7397 / 7399

页数：3

相关论文

共 31 条

[1] MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[2] Steroid-DNA interactions increasing stability, sequence-selectivity, DNA/RNA discrimination, and hypochromicity of oligonucleotide duplexes [J].

Bleczinski, CF ;

Richert, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (47) :10889-10894

[3] 5′-Tethered stilbene derivatives as fidelity- and affinity-enhancing modulators of DNA duplex stability [J].

Dogan, Z ;

Paulini, R ;

Stütz, JAR ;

Narayanan, S ;

Richert, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (15) :4762-4763

[4] A 5′-Cap for DNA Probes Binding RNA Target Strands [J].

Egetenmeyer, Simone ;

Richert, Clemens .

CHEMISTRY-A EUROPEAN JOURNAL, 2011, 17 (42) :11813-11827

[5] LNA-mediated microRNA silencing in non-human primates [J].

Elmen, Joacim ;

Lindow, Morten ;

Schutz, Sylvia ;

Lawrence, Matthew ;

Petri, Andreas ;

Obad, Susanna ;

Lindholm, Marie ;

Hedtjarn, Maj ;

Hansen, Henrik Frydenlund ;

Berger, Urs ;

Gullans, Steven ;

Kearney, Phil ;

Sarnow, Peter ;

Straarup, Ellen Marie ;

Kauppinen, Sakari .

NATURE, 2008, 452 (7189) :896-U10

[6] Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver [J].

Elmen, Joacim ;

Lindow, Morten ;

Silahtaroglu, Asli ;

Bak, Mads ;

Christensen, Mette ;

Lind-Thomsen, Allan ;

Hedtjarn, Maj ;

Hansen, Jens Bo ;

Hansen, Henrik Frydenlund ;

Straarup, Ellen Marie ;

McCullagh, Keith ;

Kearney, Phil ;

Kauppinen, Sakari .

NUCLEIC ACIDS RESEARCH, 2008, 36 (04) :1153-1162

[7]

FAKLER B, 1994, J BIOL CHEM, V269, P16187

[8] A Locked Nucleic Acid Antisense Oligonucleotide (LNA) Silences PCSK9 and Enhances LDLR Expression In Vitro and In Vivo [J].

Gupta, Nidhi ;

Fisker, Niels ;

Asselin, Marie-Claude ;

Lindholm, Marie ;

Rosenbohm, Christoph ;

Orum, Henrik ;

Elmen, Joacim ;

Seidah, Nabil G. ;

Straarup, Ellen Marie .

PLOS ONE, 2010, 5 (05)

[9] A potent 2′-O-methylated RNA-based microRNA inhibitor with unique secondary structures [J].

Haraguchi, Takeshi ;

Nakano, Haruo ;

Tagawa, Takanobu ;

Ohki, Tokimitsu ;

Ueno, Yoshihito ;

Yoshida, Tetsuo ;

Iba, Hideo .

NUCLEIC ACIDS RESEARCH, 2012, 40 (08) :e58

[10] miRNA activity directs stem cell commitment to a particular fate [J].

Hornstein, Eran .

CELL CYCLE, 2010, 9 (20) :4041-4042

← 1 2 3 4 →