Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose

被引：22

作者：

Ano, Yoshitaka ^{[1
]}

Hours, Roque A. ^{[2
]}

Akakabe, Yoshihiko ^{[3
]}

Kataoka, Naoya ^{[3
]}

Yakushi, Toshiharu ^{[3
]}

Matsushita, Kazunobu ^{[3
]}

Adachi, Osao ^{[3
]}

机构：

[1] Ehime Univ, Grad Sch Agr, Dept Biosci, Matsuyama, Ehime, Japan

[2] Natl Univ La Plata, Sch Sci, CINDEFI, La Plata, Buenos Aires, Argentina

[3] Yamaguchi Univ, Dept Biol Chem, Fac Agr, Yamaguchi, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2017年 / 81卷 / 02期

基金：

日本学术振兴会;

关键词：

acetic acid bacteria; glycerol dehydrogenase; 4-keto-D-arabonate production; 4-keto-D-pentonate; oxidative fermentation; D-GLUCONATE DEHYDROGENASE; D-GLUCOSE DEHYDROGENASE; ACETIC-ACID BACTERIA; GLUCONOBACTER-SUBOXYDANS; SORBITOL DEHYDROGENASE; PURIFICATION; FERMENTATION; 2-KETO-D-GLUCONATE; SOLUBILIZATION; INDUSTRIUS;

D O I：

10.1080/09168451.2016.1254535

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca2+. D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized D-fructose to 5-keto-D-fructose, D-psicose to 5-keto-D-psicose, including the other polyols such as, glycerol, D-ribitol, D-arabitol, and D-sorbitol. Thus, D-pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH), a major polyol dehydrogenase in Gluconobacter species. The reaction versatility of quinoprotein GLDH was notified in this study.

引用

页码：411 / 418

页数：8

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