Partitioning of β-glucosidase from Trichoderma reesei in poly(ethylene glycol) and potassium phosphate aqueous two-phase systems:: Influence of pH and temperature

被引：41

作者：

Gautam, Shalini ^{[1
]}

Simon, Laurent ^{[1
]}

机构：

[1] New Jersey Inst Technol, Otto H York Dept Chem Engn, Newark, NJ 07102 USA

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2006年 / 30卷 / 01期

关键词：

aqueous two-phase system; bioseparations; beta-D-glucosidase; protein; purification; partition coefficient;

D O I：

10.1016/j.bej.2006.02.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study evaluates the influence of pH and temperature on the partition coefficient of beta-glucosidase and total protein in order to determine the best conditions for the purification of the enzyme in poly(ethylene glycol) (PEG) 4000 (8%, w/w) and potassium phosphate salt (13%, w/w) aqueous two-phase systems. The partition coefficient increased with pH and temperature for the ranges investigated (i.e., pH: 6.0-7.5; temperature: 25-55 degrees C). Yield and selectivity were key criteria for the selection of the optimum separation condition for enzyme purification. The highest selectivity was achieved at a pH of 6.5 and a temperature of 25 degrees C. A beta-glucosidase yield greater than 92% was obtained in the bottom phase for pH values less than 7.0 and temperatures below 35 degrees C. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：104 / 108

页数：5

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