共 51 条
Pilus Phase Variation Switches Gonococcal Adherence to Invasion by Caveolin-1-Dependent Host Cell Signaling
被引:20
作者:

Faulstich, Michaela
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany

Boettcher, Jan-Peter
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany

Meyer, Thomas F.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany

Fraunholz, Martin
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany

Rudel, Thomas
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany
机构:
[1] Univ Wurzburg, Bioctr, Chair Microbiol, D-97070 Wurzburg, Germany
[2] Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany
关键词:
PROTEIN-KINASE-D;
NEISSERIA-GONORRHOEAE;
EPITHELIAL-CELLS;
N-GONORRHEA;
INFECTION;
INTERNALIZATION;
DOMAIN;
PATHOGENS;
INTERACTS;
MOLECULE;
D O I:
10.1371/journal.ppat.1003373
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Many pathogenic bacteria cause local infections but occasionally invade into the blood stream, often with fatal outcome. Very little is known about the mechanism underlying the switch from local to invasive infection. In the case of Neisseria gonorrhoeae, phase variable type 4 pili (T4P) stabilize local infection by mediating microcolony formation and inducing anti-invasive signals. Outer membrane porin PorB(IA), in contrast, is associated with disseminated infection and facilitates the efficient invasion of gonococci into host cells. Here we demonstrate that loss of pili by natural pilus phase variation is a prerequisite for the transition from local to invasive infection. Unexpectedly, both T4P-mediated inhibition of invasion and PorB(IA)-triggered invasion utilize membrane rafts and signaling pathways that depend on caveolin-1-Y14 phosphorylation (Cav1-pY14). We identified p85 regulatory subunit of PI3 kinase (PI3K) and phospholipase C gamma 1 as new, exclusive and essential interaction partners for Cav1-pY14 in the course of PorB(IA)-induced invasion. Active PI3K induces the uptake of gonococci via a new invasion pathway involving protein kinase D1. Our data describe a novel route of bacterial entry into epithelial cells and offer the first mechanistic insight into the switch from local to invasive gonococcal infection.
引用
收藏
页数:16
相关论文
共 51 条
[1]
Mutagenesis of the Neisseria gonorrhoeae porin reduces invasion in epithelial cells and enhances phagocyte responsiveness
[J].
Bauer, FJ
;
Rudel, T
;
Stein, M
;
Meyer, TF
.
MOLECULAR MICROBIOLOGY,
1999, 31 (03)
:903-913

Bauer, FJ
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany

Rudel, T
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany

Stein, M
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany

Meyer, TF
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany
[2]
Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways
[J].
Billker, O
;
Popp, A
;
Brinkmann, V
;
Wenig, G
;
Schneider, J
;
Caron, E
;
Meyer, TF
.
EMBO JOURNAL,
2002, 21 (04)
:560-571

Billker, O
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Popp, A
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Brinkmann, V
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Wenig, G
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Schneider, J
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Caron, E
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany

Meyer, TF
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany
[3]
Contribution of interactions between complement inhibitor C4b-binding protein and pathogens to their ability to establish infection with particular emphasis on Neisseria gonorrhoeae
[J].
Blom, Anna M.
;
Ram, Sanjay
.
VACCINE,
2008, 26
:I49-I55

Blom, Anna M.
论文数: 0 引用数: 0
h-index: 0
机构:
Lund Univ, Dept Lab Med, Div Med Prot Chem, S-20502 Malmo, Sweden Lund Univ, Dept Lab Med, Div Med Prot Chem, S-20502 Malmo, Sweden

论文数: 引用数:
h-index:
机构:
[4]
Tyrosine-Phosphorylated Caveolin-1 Blocks Bacterial Uptake by Inducing Vav2-RhoA-Mediated Cytoskeletal Rearrangements
[J].
Boettcher, Jan Peter
;
Kirchner, Marieluise
;
Churin, Yuri
;
Kaushansky, Alexis
;
Pompaiah, Malvika
;
Thorn, Hans
;
Brinkmann, Volker
;
MacBeath, Gavin
;
Meyer, Thomas F.
.
PLOS BIOLOGY,
2010, 8 (08)
:55-56

Boettcher, Jan Peter
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Kirchner, Marieluise
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Churin, Yuri
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Kaushansky, Alexis
论文数: 0 引用数: 0
h-index: 0
机构:
Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Pompaiah, Malvika
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Thorn, Hans
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Brinkmann, Volker
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

MacBeath, Gavin
论文数: 0 引用数: 0
h-index: 0
机构:
Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

Meyer, Thomas F.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany
[5]
Phosphatidylinositol 3-kinases in carcinoembryonic antigen-related cellular adhesion molecule-mediated internalization of Neisseria gonorrhoeae
[J].
Booth, JW
;
Telio, D
;
Liao, EH
;
McCaw, SE
;
Matsuo, T
;
Grinstein, S
;
Gray-Owen, SD
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2003, 278 (16)
:14037-14045

Booth, JW
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

Telio, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

Liao, EH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

McCaw, SE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

Matsuo, T
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

Grinstein, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

Gray-Owen, SD
论文数: 0 引用数: 0
h-index: 0
机构: Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada
[6]
GONOCOCCAL-INFECTION - A MODEL OF MOLECULAR PATHOGENESIS
[J].
BRITIGAN, BE
;
COHEN, MS
;
SPARLING, PF
.
NEW ENGLAND JOURNAL OF MEDICINE,
1985, 312 (26)
:1683-1694

BRITIGAN, BE
论文数: 0 引用数: 0
h-index: 0
机构: UNIV N CAROLINA, SCH MED, DEPT MED, CHAPEL HILL, NC 27514 USA

COHEN, MS
论文数: 0 引用数: 0
h-index: 0
机构: UNIV N CAROLINA, SCH MED, DEPT MED, CHAPEL HILL, NC 27514 USA

SPARLING, PF
论文数: 0 引用数: 0
h-index: 0
机构: UNIV N CAROLINA, SCH MED, DEPT MED, CHAPEL HILL, NC 27514 USA
[7]
Phosphatidylinositol 3′-Kinase Activity Is Critical for Initiating the Oxidative Burst and Bacterial Destruction during CEACAM3-mediated Phagocytosis
[J].
Buntru, Alexander
;
Kopp, Kathrin
;
Voges, Maike
;
Frank, Ronald
;
Bachmann, Verena
;
Hauck, Christof R.
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2011, 286 (11)
:9555-9566

Buntru, Alexander
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany
Univ Konstanz, Konstanz Res Sch Chem Biol, D-78457 Constance, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany

Kopp, Kathrin
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany

Voges, Maike
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany
Univ Konstanz, Konstanz Res Sch Chem Biol, D-78457 Constance, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany

Frank, Ronald
论文数: 0 引用数: 0
h-index: 0
机构:
Helmholtz Ctr Infect Res, Dept Biol Chem, D-38124 Braunschweig, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany

Bachmann, Verena
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany

Hauck, Christof R.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany
Univ Konstanz, Konstanz Res Sch Chem Biol, D-78457 Constance, Germany Univ Konstanz, Lehrstuhl Zellbiol, D-78457 Constance, Germany
[8]
Differential expression and function of caveolin-1 in human gastric cancer progression
[J].
Burgermeister, Elke
;
Xing, Xiangbin
;
Roecken, Christoph
;
Juhasz, Mark
;
Chen, Jie
;
Hiber, Michaela
;
Mair, Katrin
;
Shatz, Maria
;
Liscovitch, Moti
;
Schmid, Roland M.
;
Ebert, Matthias P. A.
.
CANCER RESEARCH,
2007, 67 (18)
:8519-8526

Burgermeister, Elke
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Xing, Xiangbin
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Roecken, Christoph
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Juhasz, Mark
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Chen, Jie
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Hiber, Michaela
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Mair, Katrin
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

论文数: 引用数:
h-index:
机构:

Liscovitch, Moti
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Schmid, Roland M.
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany

Ebert, Matthias P. A.
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Munich, Klinikum Rechts Isar, Dept Med 2, D-81675 Munich, Germany
[9]
CONFIRMATION OF ASSOCIATION OF PROTEIN-I SEROTYPE OF NEISSERIA-GONORRHOEAE WITH ABILITY TO CAUSE DISSEMINATED INFECTION
[J].
CANNON, JG
;
BUCHANAN, TM
;
SPARLING, PF
.
INFECTION AND IMMUNITY,
1983, 40 (02)
:816-819

CANNON, JG
论文数: 0 引用数: 0
h-index: 0
机构:
SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114 SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114

BUCHANAN, TM
论文数: 0 引用数: 0
h-index: 0
机构:
SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114 SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114

SPARLING, PF
论文数: 0 引用数: 0
h-index: 0
机构:
SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114 SEATTLE PUBL HLTH HOSP,IMMUNOL RES LAB,SEATTLE,WA 98114
[10]
SUMOylation of the GTPase Rac1 is required for optimal cell migration
[J].
Castillo-Lluva, Sonia
;
Tatham, Michael H.
;
Jones, Richard C.
;
Jaffray, Ellis G.
;
Edmondson, Ricky D.
;
Hay, Ronald T.
;
Malliri, Angeliki
.
NATURE CELL BIOLOGY,
2010, 12 (11)
:1078-U70

Castillo-Lluva, Sonia
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Tatham, Michael H.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dundee, Wellcome Trust Ctr Gene Regulat & Express, Sir James Black Ctr Coll Life Sci, Dundee DD1 5EH, Scotland Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Jones, Richard C.
论文数: 0 引用数: 0
h-index: 0
机构:
US FDA, NCTR, Jefferson, AR 72079 USA Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Jaffray, Ellis G.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dundee, Wellcome Trust Ctr Gene Regulat & Express, Sir James Black Ctr Coll Life Sci, Dundee DD1 5EH, Scotland Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Edmondson, Ricky D.
论文数: 0 引用数: 0
h-index: 0
机构:
US FDA, NCTR, Jefferson, AR 72079 USA Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Hay, Ronald T.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dundee, Wellcome Trust Ctr Gene Regulat & Express, Sir James Black Ctr Coll Life Sci, Dundee DD1 5EH, Scotland Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Malliri, Angeliki
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Univ Manchester, Cell Signalling Grp, Canc Res UK Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England