Fabry-Perot fiber-optic immunosensor based on suspended layer-by-layer (chitosan/polystyrene sulfonate) membrane

被引：106

作者：

Chen, L. H. ^{[1
]}

Chan, C. C. ^{[1
]}

Menon, R. ^{[1
]}

Balamurali, P. ^{[1
]}

Wong, W. C. ^{[1
]}

Ang, X. M. ^{[3
]}

Hu, P. B. ^{[1
,2
]}

Shaillender, M. ^{[5
]}

Neu, B. ^{[6
]}

Zu, P. ^{[1
]}

Tou, Z. Q. ^{[1
]}

Poh, C. L. ^{[1
]}

Leong, K. C. ^{[4
]}

机构：

[1] Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 63754, Singapore

[2] China Jiliang Univ, Inst Optoelect Technol, Hangzhou 310018, Zhejiang, Peoples R China

[3] Natl Univ Singapore, Sch Integrat Sci & Engn NGS, Singapore 117456, Singapore

[4] GLOBALFOUNDRIES Singapore Pte Ltd, Singapore 738406, Singapore

[5] Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 637457, Singapore

[6] Rhine Waal Univ Appl Sci, Fac Life Sci, Kleve, Germany

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2013年 / 188卷

关键词：

Fabry-Perot interferometer; Layer-by-layer self assembly; Immunosensor; Biosensor; CHITOSAN THIN-FILMS; OPTICAL BIOSENSORS; SENSOR; AFM;

D O I：

10.1016/j.snb.2013.06.093

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free fiber-optic Fabry-Perot interferometric (FPI) immunosensor is proposed by layer-by-layer (LBL) self-assembly of a suspended chitosan and polystyrene sulfonate membrane at the tip of a hollow fiber. Immunoglobulin G (IgG) was immobilized on the polyelectrolyte substrate and the detection of target anti-immunoglobulin G (anti-IgG) was monitored through the measurement of substrate's effective optical thickness. The sensitivity and specificity of the sensor were studied using correlated and non-correlated anti-IgG/IgG pair. Sensitivity of 0.033 mu m/(pg/mm(2)) and limit of detection of 0.005 nM was achieved with the sensor displaying minimal response toward non-correlated anti-IgG. The results demonstrate the feasibility of the sensor for immunosensing. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：185 / 192

页数：8

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