Low pH D-xylonate production with Pichia kudriavzevii

被引：69

作者：

Toivari, Mervi ^{[1
]}

Vehkomaki, Maija-Leena ^{[1
]}

Nygard, Yvonne ^{[1
]}

Penttila, Merja ^{[1
]}

Ruohonen, Laura ^{[1
]}

Wiebe, Marilyn G. ^{[1
]}

机构：

[1] VTT, Tech Res Ctr Finland, FI-02044 Espoo, Finland

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 133卷

基金：

芬兰科学院;

关键词：

Pichia kudriavzevii; Issatchenkia orientalis; D-Xylonate; D-Xylose; Low pH; LACTIC-ACID PRODUCTION; D-XYLOSE METABOLISM; SACCHAROMYCES-CEREVISIAE; ISSATCHENKIA-ORIENTALIS; MICROBIAL-PRODUCTION; CONTINUOUS-CULTURE; TOLERANCE; ETHANOL; YEAST;

D O I：

10.1016/j.biortech.2013.01.157

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

D-Xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VU C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171 g L-1 of D-xylonate from 171 g L-1 D-xylose at a rate of 1.4 g L-1 h(-1) and yield of 1.0 g [g substrate consumed](-1), which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146 g L-1 D-xylonate at 1.2 g L-1 h(-1) at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：555 / 562

页数：8

共 35 条

[1] Technology development for the production of biobased products from biorefinery carbohydrates-the US Department of Energy's "Top 10" revisited [J].